ArticleProceedings of the National Academy of Sciences of the United States of America2021
Establishment of bovine expanded potential stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 96 citations in OpenAlex.
- Livestock Blastoids: In Vitro Blastocyst Models for Embryogenesis and Reproductive Technologies.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Suidae iPSC-derived macrophages as models for investigating susceptibility and resilience to African swine fever virus.Stem cell reports · 2026Article
- Recent Advances in Cryopreservation of Animal Genetic Resources: Principles, Influencing Factors, and Material-Specific Challenges.Animals : an open access journal from MDPI · 2026Review
- A universal 6iL/E4 culture system for deriving and maintaining embryonic stem cells across mammalian species.Cell research · 2026Article
- Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells.Nature biotechnology · 2026Article
- Generation of Functional GnRH-Responsive Pituitary-like Organoids from Ovine Embryonic Stem Cells.Cells · 2026Article
- A feeder-free culture system supports long-term expansion and germline competence of bovine formative embryonic stem cells†.Biology of reproduction · 2026Article
- Low oxygen environment alters transcripts related to energy metabolism without altering the pluripotency core of bovine embryonic stem cells.Biology open · 2026Article
- Efficient derivation of stable sheep embryonic stem cells opens a new avenue for agricultural and biomedical application.Journal of advanced research · 2026Article
- Derivation of Embryonic Stem Cells from an Endangered Cattle Breed via Somatic Cell Nuclear Transfer.Cells · 2026Article
- Effects of leonurine supplementation during in vitro culture on oxidative stress, cell proliferation, apoptosis, and autophagy in bovine embryos.Scientific reports · 2026Article
- Wnt/β-Catenin Activation by iCRT3 Enhanced the Pluripotency of Bovine Expanded Pluripotent Stem Cells.Animals : an open access journal from MDPI · 2026Article
- Comparative transcriptomic analysis of embryonic stem cells across mammalian species.iScience · 2026Article
- Developing stem cell-based bovine embryos for in vitro breeding.The Journal of reproduction and development · 2026Review
- Stem Cells and Wildlife Conservation.Advances in experimental medicine and biology · 2026Review
- Novel feeder-cell-free 3C system promotes the transition from primed to formative pluripotency, self-renewal, and germline differentiation in rabbit embryonic stem cellsFrontiers in cell and developmental biology · 2026Article
- Transcriptomic insights and feeder-free culturing of porcine expanded potential stem cells from cloned embryos.Stem cell research & therapy · 2025Article
- Advances in the Study of Pluripotent Stem Cells in Livestock.Cell proliferation · 2025Review
- Scaling Cultured Meat: Challenges and Solutions for Affordable Mass Production.Comprehensive reviews in food science and food safety · 2025Review
- Morula complementation restores male germline inPNAS nexus · 2025Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
23 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eight-cell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.