ArticleAJNR. American journal of neuroradiology2021
Novel Human Acute Ischemic Stroke Blood Clot Analogs for In Vitro Thrombectomy Testing.
Article in AJNR. American journal of neuroradiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 27 citations in OpenAlex.
- A multi-omics approach investigating thrombolysis resistance in acute ischemic stroke thrombi.Journal of thrombosis and thrombolysis · 2026Article
- The modified placenta model for microsurgery training: Recommended methods for comprehensive microsurgery experience.Journal of hand and microsurgery · 2024Article
- Contribution of Red Blood Cells and Platelets to Blood Clot Computed Tomography Imaging and Compressive Mechanical Characteristics.Annals of biomedical engineering · 2024Article
- MicroCT and Histological Analysis of Clot Composition in Acute Ischemic Stroke : A Comparative Study of MT-Retrieved Clots and Clot Analogs.Clinical neuroradiology · 2024Article
- Dynamic Perviousness Predicts Revascularization Success in Acute Ischemic Stroke.Diagnostics (Basel, Switzerland) · 2024Article
- Microfabricated Nitinol Stent Retrievers with a Micro-Patterned Surface.Micromachines · 2024Article
- In vitro models for the experimental evaluation of mechanical thrombectomy devices in acute ischemic stroke.Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences · 2023Review
- Tensile and Compressive Mechanical Behaviour of Human Blood Clot Analogues.Annals of biomedical engineering · 2023Article
- An in vitro model for Extracellular DNA Traps (ETs)-rich Human Thrombus Analogs.Journal of neurointerventional surgery · 2023Article
- Construction and Validation of a Benchtop Model for Testing of Mechanical Thrombectomy Devices for Pulmonary Embolism.Cardiovascular and interventional radiology · 2023Article
- Experimental Validation of Pullout Resistance for Stent Retrievers and Aspiration Catheters.Journal of neuroendovascular therapy · 2022Article
- MicroCT Can Characterize Clots Retrieved With Mechanical Thrombectomy From Acute Ischemic Stroke Patients-A Preliminary Report.Frontiers in neurology · 2022Article
- A Thrombectomy Model Based on Ex Vivo Whole Human Brains.AJNR. American journal of neuroradiology · 2021Article
- Multimodality Characterization of the Clot in Acute Stroke.Frontiers in neurology · 2021Article
- Unmet Needs in Acute Ischemic Stroke: Overcoming Recalcitrant Clots.Cardiology in reviewReview
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
background and purposePrevious studies have successfully created blood clot analogs for in vitro endovascular device testing using animal blood of various species. Blood components vary greatly among species; therefore, creating clot analogs from human blood is likely a more accurate representation of thrombi formed in the human vasculature. MATERIALS AND
methodsFollowing approval from the Mayo Clinic institutional review board, human whole-blood and platelet donations were obtained from the blood transfusion service. Twelve clot analogs were created by combining different ratios of red blood cells + buffy coat, plasma, and platelets. Thrombin and calcium chloride were added to stimulate coagulation. Clot composition was assessed using histologic and immunohistochemical staining. To assess the similarities of mechanical properties to patient clots, 3 types of clot analogs (soft, elastic, and stiff) were selected for in vitro thrombectomy testing.
resultsThe range of histopathologic compositions produced is representative of clots removed during thrombectomy procedures. The red blood cell composition ranged from 8.9% to 91.4%, and fibrin composition ranged from 3.1% to 53.4%. Platelets (CD42b) and von Willebrand Factor ranged from 0.5% to 47.1% and 1.0% to 63.4%, respectively. The soft clots had the highest first-pass effect and successful revascularization rates followed by the elastic and stiff clots. Distal embolization events were observed when clot ingestion could not be achieved, requiring device pullback. The incidence rate of distal embolization was the highest for the stiff clots due to the weak clot/device integration.
conclusionsRed blood cell-rich, fibrin-rich, and platelet-rich clot analogs that mimic clots retrieved from patients with acute ischemic stroke were created in vitro. Differing retrieval outcomes were confirmed using in vitro thrombectomy testing in a subset of clots.
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