ArticlePLoS genetics2021
Sperm acrosome overgrowth and infertility in mice lacking chromosome 18 pachytene piRNA.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 74 citations in OpenAlex.
- Spatiotemporal control of PIWI compartmentalization by mitochondrial scaffolds defines pachytene piRNA pathway organization.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Divergent regulatory impacts of endogenous siRNAs on host mRNAs in testis of closely related species.bioRxiv : the preprint server for biology · 2026Article
- Article
- Testis-specific lncRNA Teshl regulates acrosome biogenesis to maintain sperm structure and function.Cell & bioscience · 2026Article
- Positive Selection Targeted Primate Genes that Encode Transposable Element Repressors.Genome biology and evolution · 2026Article
- Biogenesis and migration of the sperm cytoplasmic droplet require ARRDC5 and TEX38.Cell reports · 2026Article
- Article
- Spatiotemporal control of PIWI compartmentalization by mitochondrial scaffolds defines pachytene piRNA pathway organization.bioRxiv : the preprint server for biology · 2025Article
- Exploring the Characters of Non-Coding RNAs in Spermatogenesis and Male Infertility.International journal of molecular sciences · 2025Review
- Cracking the code: how piRNA pathway shapes spermatogenesis and combats male infertility.Frontiers in cell and developmental biology · 2025Review
- Mouse Pachytene piRNAs Cleave Hundreds of Transcripts, But Alter the Steady-State Abundance of Only a Minority of Targets.bioRxiv : the preprint server for biology · 2024Article
- PIWI-interacting RNAs: who, what, when, where, why, and how.The EMBO journal · 2024Article
- RNA helicase D1PAS1 resolves R-loops and forms a complex for mouse pachytene piRNA biogenesis required for male fertility.Nucleic acids research · 2024Article
- A comparative roadmap of PIWI-interacting RNAs across seven species reveals insights into de novo piRNA-precursor formation in mammals.Cell reports · 2024Article
- Enigmatic Pachytene PIWI-Interacting RNAs.Genome biology and evolution · 2024Review
- PNLDC1 catalysis and postnatal germline function are required for piRNA trimming, LINE1 silencing, and spermatogenesis in mice.PLoS genetics · 2024Article
- Mammalian PIWI-piRNA-target complexes reveal features for broad and efficient target silencing.Nature structural & molecular biology · 2024Article
- Heat stress induced piRNA alterations in pachytene spermatocytes and round spermatids.Reproductive biology and endocrinology : RB&E · 2024Article
- MIWI N-terminal arginines orchestrate generation of functional pachytene piRNAs and spermiogenesis.Nucleic acids research · 2024Article
- Testicular piRNA Analysis Identified Dysregulated piRNAs in Non-obstructive Azoospermia.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
piRNAs are small non-coding RNAs required to maintain genome integrity and preserve RNA homeostasis during male gametogenesis. In murine adult testes, the highest levels of piRNAs are present in the pachytene stage of meiosis, but their mode of action and function remain incompletely understood. We previously reported that BTBD18 binds to 50 pachytene piRNA-producing loci. Here we show that spermatozoa in gene-edited mice lacking a BTBD18 targeted pachytene piRNA cluster on Chr18 have severe sperm head dysmorphology, poor motility, impaired acrosome exocytosis, zona pellucida penetration and are sterile. The mutant phenotype arises from aberrant formation of proacrosomal vesicles, distortion of the trans-Golgi network, and up-regulation of GOLGA2 transcripts and protein associated with acrosome dysgenesis. Collectively, our findings reveal central role of pachytene piRNAs in controlling spermiogenesis and male fertility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.