Evidence map›Paper›PMID 33829634›Full record

ArticleJournal of thrombosis and haemostasis : JTH2021

Gene and protein expression in human megakaryocytes derived from induced pluripotent stem cells.

Kai Kammers, Margaret A Taub, Rasika A Mathias, Lisa R Yanek, Kanika Kanchan, Vidya Venkatraman, Niveda Sundararaman, Joshua Martin, Senquan Liu, Dixie Hoyle and 11 more

Open access · hybridAbstract read
PubMed Publisher
In one paragraph

Article in Journal of thrombosis and haemostasis : JTH, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 10 citations in OpenAlex.

  1. Integrated single-cell transcriptomic analysis identifiesBiochemistry and biophysics reports · 2026
    Article
  2. Production of plateletsHaematologica · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. iPSC diversity: A key for better use and improved targeting.Journal of thrombosis and haemostasis : JTH · 2021
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

21 authors at 5 institutions in 1 country.

Kai KammersDivision of Biostatistics and Bioinformatics, Department of Oncology, Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Margaret A TaubDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Rasika A MathiasThe GeneSTAR Program, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Lisa R YanekThe GeneSTAR Program, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Kanika KanchanDivision of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Vidya VenkatramanAdvanced Clinical Biosystems Research Institute, Barbra Streisand Woman's Heart Center, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Niveda SundararamanAdvanced Clinical Biosystems Research Institute, Barbra Streisand Woman's Heart Center, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Joshua MartinThe GeneSTAR Program, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Senquan LiuDivision of Hematology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Dixie HoyleDivision of Hematology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Koen RaedscheldersAdvanced Clinical Biosystems Research Institute, Barbra Streisand Woman's Heart Center, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Ronald HolewinskiAdvanced Clinical Biosystems Research Institute, Barbra Streisand Woman's Heart Center, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Sarah ParkerAdvanced Clinical Biosystems Research Institute, Barbra Streisand Woman's Heart Center, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Victoria DardovAdvanced Clinical Biosystems Research Institute, Barbra Streisand Woman's Heart Center, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Nauder FaradayThe GeneSTAR Program, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Diane M BeckerThe GeneSTAR Program, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Linzhao ChengDivision of Hematology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Zack Z WangDivision of Hematology and Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Jeffrey T LeekDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA.
Jennifer E Van EykAdvanced Clinical Biosystems Research Institute, Barbra Streisand Woman's Heart Center, The Smidt Heart Institute, Cedars-Sinai Medical Center, Los Angeles, California, USA.
Lewis C BeckerThe GeneSTAR Program, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Johns Hopkins Medicine · USJohns Hopkins University · USCedars-Sinai Smidt Heart Institute · USCedars-Sinai Medical Center · USSidney Kimmel Comprehensive Cancer Center · US

Funding

Translational Research Central ServicesP30CA006973 · NCI · JOHNS HOPKINS UNIVERSITY · PI ALAN KEITH MEEKER · 1985 to 2026
$208.6M
Genomic and Proteomic Architecture of AtherosclerosisR01HL111362 · NHLBI · WAKE FOREST UNIVERSITY HEALTH SCIENCES · PI HERRINGTON, DAVID MCLEOD · 2012 to 2021
$17.2M
Genotypic Determinants of Aspirin Response in High Risk FamiliesU01HL072518 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI BECKER, LEWIS C · 2002 to 2008
$12.8M
Functional Genomics of Platelet Aggregation Using iPS and Derived MegakaryocytesU01HL107446 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI BECKER, LEWIS C, CHENG, LINZHAO · 2011 to 2015
$8.8M
A Family-based Exome Sequencing Approach to Identify Platelet Aggregation GenesR01HL112064 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI MATHIAS, RASIKA ANN · 2012 to 2015
$5.1M
Statistical models for biological and technical variation in RNA sequencingR01GM105705 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI LEEK, JEFFREY T. · 2013 to 2017
$1.5M
NCI NIH HHS P30-CA006973NHLBI NIH HHS HHSN268201100037CNHLBI NIH HHS R01 HL111362NHLBI NIH HHS R01 HL112064NHLBI NIH HHS U01 HL107446NHLBI NIH HHS U01 HL72518NIH HHS R01 GM105705
6 · The paper itself

Abstract

backgroundThere is interest in deriving megakaryocytes (MKs) from pluripotent stem cells (iPSC) for biological studies. We previously found that genomic structural integrity and genotype concordance is maintained in iPSC-derived MKs.

objectiveTo establish a comprehensive dataset of genes and proteins expressed in iPSC-derived MKs.

methodsiPSCs were reprogrammed from peripheral blood mononuclear cells (MNCs) and MKs were derived from the iPSCs in 194 healthy European American and African American subjects. mRNA was isolated and gene expression measured by RNA sequencing. Protein expression was measured in 62 of the subjects using mass spectrometry. RESULTS AND

conclusionsMKs expressed genes and proteins known to be important in MK and platelet function and demonstrated good agreement with previous studies in human MKs derived from CD34+ progenitor cells. The percent of cells expressing the MK markers CD41 and CD42a was consistent in biological replicates, but variable across subjects, suggesting that unidentified subject-specific factors determine differentiation of MKs from iPSCs. Gene and protein sets important in platelet function were associated with increasing expression of CD41/42a, while those related to more basic cellular functions were associated with lower CD41/42a expression. There was differential gene expression by the sex and race (but not age) of the subject. Numerous genes and proteins were highly expressed in MKs but not known to play a role in MK or platelet function; these represent excellent candidates for future study of hematopoiesis, platelet formation, and/or platelet function.

Indexed as

Induced Pluripotent Stem CellsBlood PlateletsCell DifferentiationGenomicsHumansLeukocytes, MononuclearMegakaryocytesgene expressioninduced pluripotent stem cellsmass spectrometrymegakaryocytesplatelets

Identifiers

PMID33829634
OpenAlexW3148520390

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.