ArticleScientific reports2021
Reference gene selection for expression studies in the reproductive axis tissues of Magang geese at different reproductive stages under light treatment.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 19 citations in OpenAlex.
- Integrative WGBS and ATAC-seq profiling reveals epigenetic and chromatin accessibility signatures associated with clutch length in goose ovaries.Poultry science · 2026Article
- Enhanced endocrine-metabolic support and axonemal assembly in high-sperm-motility geese: insights from testicular cellular heterogeneity by scRNA-seq.Poultry science · 2026Article
- Expression pattern ofJournal of advanced veterinary and animal research · 2026Article
- Investigation of the effect of tannic acid on doxorubicin-ınduced testicular damage and functions in a rat model.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Gene Expression of Feed Intake-Regulating Peptides in the Gut-Brain Axis of Laying Hens Housed Under Two Different Egg Production Systems.Animals : an open access journal from MDPI · 2025Article
- Article
- Glucuronolactone Restores the Intestinal Barrier and Redox Balance Partly Through the Nrf2/Akt/FOXO1 Pathway to Alleviate Weaning Stress-Induced Intestinal Dysfunction in Piglets.Antioxidants (Basel, Switzerland) · 2025Article
- Screening of Reference Genes for Quantitative Real-Time PCR Analysis in Tissues and during Testis Development, and Application to Analyze the Expression ofAnimals : an open access journal from MDPI · 2024Article
- Expression of housekeeping genes varies depending on mevalonate pathway inhibition in cancer cells.Heliyon · 2023Article
- Identification of reference genes for expression studies in the liver and spleen of laying hens housed in cage and cage-free systems.Open veterinary journal · 2023Article
- Reliable reference genes and abiotic stress marker genes in Klebsormidium nitens.Scientific reports · 2022Article
- Evaluation of Reference Genes in the Polyploid ComplexPlants (Basel, Switzerland) · 2022Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In quantitative PCR research, appropriate reference genes are key to determining accurate mRNA expression levels. In order to screen the reference genes suitable for detecting gene expression in tissues of the reproductive axis, a total of 420 (males and females = 1:5) 3-year-old Magang geese were selected and subjected to light treatment. The hypothalamus, pituitary and testicular tissues were subsequently collected at different stages. Ten genes including HPRT1, GAPDH, ACTB, LDHA, SDHA, B2M, TUBB4, TFRC, RPS2 and RPL4 were selected as candidate reference genes. The expression of these genes in goose reproductive axis tissues was detected by real-time fluorescent quantitative PCR. The ΔCT, geNorm, NormFinder and BestKeeper algorithms were applied to sort gene expression according to stability. The results showed that ACTB and TUBB4 were the most suitable reference genes for the hypothalamic tissue of Magang goose in the three breeding stages; HPRT1 and RPL4 for pituitary tissue; and HPRT1 and LDHA for testicular tissue. For all three reproductive axis tissues, ACTB was the most suitable reference gene, whereas the least stable reference gene was GAPDH. Altogether, these results can provide references for tissue expression studies in geese under light treatment.
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