ArticleGenome biology2021
ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics.
Article in Genome biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
67 citing papers in PubMed.
- Multiplexed gene expression analysis in dissociated and intact planarian flatworm tissues using in situ hybridization chain reaction.Biology open · 2026Article
- Multi-stage single-cell atlas of a chordate non-embryonic development unveils dedifferentiating bud founder cells.Nature communications · 2026Article
- A gene-agnostic FACS technique to isolate stem cells inbioRxiv : the preprint server for biology · 2026Article
- Notch coordinates self-organization of germ layers and axial polarity in sea anemone gastruloids.Nature communications · 2026Article
- Tissue rigidity phase transition shapes morphogen gradients.Nature cell biology · 2026Article
- Two neuropeptides that promote blood feeding ineLife · 2026Article
- Functional characterization of specialized immune cells in a cnidarian reveals an ancestral antiviral program.Nature communications · 2026Article
- A Field-Friendly, Non-Toxic Fixative for Integrated Morphological and Molecular Research in Non-Model Invertebrates.Ecology and evolution · 2026Article
- Metabolites produced by agat+ cells support regeneration in the planarian Schmidtea mediterranea.Developmental biology · 2026Article
- Decoding cnidarian cell type gene regulation.Nature ecology & evolution · 2026Article
- Methodologies for Sample Multiplexing and Computational Deconvolution in Single-Cell Sequencing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- The nascent RNA labelling compound 5-ethynyl uridine (EU) integrates into DNA in some animals.BMC molecular and cell biology · 2025Article
- An updated and spatially validated somatic single-cell atlas of Hydractinia symbiolongicarpus.BMC genomics · 2025Article
- Article
- Light-modulated stem cells in the camera-type eye of an annelid model for adult brain plasticity.Nature communications · 2025Article
- Multimodal single cell analyses reveal gene networks of planarian stem cell differentiation.Nature communications · 2025Article
- Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis: insights into tissue specialization and blastogenesis.Development (Cambridge, England) · 2025Article
- How Single-Cell Transcriptomics of Hydractinia Is Informing the Evolution of Cnidarian Sensory Systems.Integrative and comparative biology · 2025Review
- Establishing single cell RNA transcriptomics: a brief guide.Frontiers in zoology · 2025Review
- Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
Abstract
Single-cell sequencing technologies are revolutionizing biology, but they are limited by the need to dissociate live samples. Here, we present ACME (ACetic-MEthanol), a dissociation approach for single-cell transcriptomics that simultaneously fixes cells. ACME-dissociated cells have high RNA integrity, can be cryopreserved multiple times, and are sortable and permeable. As a proof of principle, we provide single-cell transcriptomic data of different species, using both droplet-based and combinatorial barcoding single-cell methods. ACME uses affordable reagents, can be done in most laboratories and even in the field, and thus will accelerate our knowledge of cell types across the tree of life.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.