ArticleMicrobial cell factories2021
Trehalose alleviates high-temperature stress in Pleurotus ostreatus by affecting central carbon metabolism.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Heat stress-induced degradation of glutamine synthetase rebalances central carbon-nitrogen metabolism and promotes thermotolerance inApplied and environmental microbiology · 2026Article
- Glutathione peroxidase gene regulates heat stress response via trehalose synthesis in Pleurotus ostreatus.Applied microbiology and biotechnology · 2026Article
- Evolutionary Analysis of Trehalose Breakdown Pathways.Genome biology and evolution · 2026Article
- Unraveling the Signaling Networks: How Exogenous Substances Mitigate Heat Stress in Edible Fungi.Journal of fungi (Basel, Switzerland) · 2026Review
- Beyond antibiotics: a new anti-virulence strategy combating ESBL-producing Klebsiella pneumoniae by targeting periplasmic trehalase.BMC microbiology · 2026Article
- GSK3 phosphorylates and activates trehalose-6-phosphate synthase to improve trehalose production and thermotolerance in Ganoderma lucidum.Communications biology · 2025Article
- Transcriptome Analysis Reveals Critical Genes Involved in the Response ofCurrent issues in molecular biology · 2025Article
- Growth Phase-Dependent Changes in the Carbohydrate Metabolism of Penicillium Strains from Diverse Temperature Classes in Response to Cold Stress.International journal of molecular sciences · 2025Article
- Physiological Response ofInsects · 2025Article
- Comparative transcriptome analysis reveals the role of sugar signaling in response to high temperature stress in Armillaria gallica.BMC microbiology · 2025Article
- Combined Physiological and Transcriptomic Analyses of the Effects of Exogenous Trehalose on Salt Tolerance in Maize (Plants (Basel, Switzerland) · 2024Article
- The Promoting Mechanism of the Sterile Fermentation Filtrate ofBiomolecules · 2023Article
- Combined transcriptomics and metabolomics analysis reveals the molecular mechanism of heat tolerance of Le023M, a mutant inHeliyon · 2023Article
- Salicylic Acid Treatment Alleviates the Heat Stress Response by Reducing the Intracellular ROS Level and Increasing the Cytosolic Trehalose Content in Pleurotus ostreatus.Microbiology spectrum · 2023Article
- Analysis and modification of central carbon metabolism inFrontiers in microbiology · 2023Article
- Integrating LC-MS and HS-GC-MS for the metabolite characterization of the Chinese medicinal plantFrontiers in nutrition · 2023Article
- Improving the productivity of malic acid by alleviating oxidative stress during Aspergillus niger fermentation.Biotechnology for biofuels and bioproducts · 2022Article
- Salicylic Acid Enhances Heat Stress Resistance ofAntioxidants (Basel, Switzerland) · 2022Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundTrehalose, an intracellular protective agent reported to mediate defense against many stresses, can alleviate high-temperature-induced damage in Pleurotus ostreatus. In this study, the mechanism by which trehalose relieves heat stress was explored by the addition of exogenous trehalose and the use of trehalose-6-phosphate synthase 1 (tps1) overexpression transformants.
resultsThe results suggested that treatment with exogenous trehalose or overexpression of tps1 alleviated the accumulation of lactic acid under heat stress and downregulated the expression of the phosphofructokinase (pfk) and pyruvate kinase (pk) genes, suggesting an ameliorative effect of trehalose on the enhanced glycolysis in P. ostreatus under heat stress. However, the upregulation of hexokinase (hk) gene expression by trehalose indicated the involvement of the pentose phosphate pathway (PPP) in heat stress resistance. Moreover, treatment with exogenous trehalose or overexpression of tps1 increased the gene expression level and enzymatic activity of glucose-6-phosphate dehydrogenase (g6pdh) and increased the production of both the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) and glutathione (GSH), confirming the effect of trehalose on alleviating oxidative damage by enhancing PPP in P. ostreatus under heat stress. Furthermore, treatment with exogenous trehalose or overexpression of tps1 ameliorated the decrease in the oxygen consumption rate (OCR) caused by heat stress, suggesting a relationship between trehalose and mitochondrial function under heat stress.
conclusionsTrehalose alleviates high-temperature stress in P. ostreatus by inhibiting glycolysis and stimulating PPP activity. This study may provide further insights into the heat stress defense mechanism of trehalose in edible fungi from the perspective of intracellular metabolism.
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