ArticleCell reports2021
Restoration of fitness lost due to dysregulation of the pyruvate dehydrogenase complex is triggered by ribosomal binding site modifications.
Article in Cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 27 citations in OpenAlex.
- Meta-analysis Driven Strain Design for Mitigating Oxidative Stresses Important in Biomanufacturing.ACS synthetic biology · 2024Pooled it
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- C6‑HSL enhances BPA tolerance inCurrent research in microbial sciences · 2026Article
- Deciphering the Impact of Temperature on Pleiotropic Consequences of RNA Polymerase Mutations.Molecular biology and evolution · 2025Article
- Laboratory Evolution Reveals Transcriptional Mechanisms Underlying Thermal Adaptation of Escherichia coli.Genome biology and evolution · 2025Article
- Naphthoquinone preferentially pairs with non-proton-pumping NADH dehydrogenase for respiratory electron transport.PLoS genetics · 2025Article
- Diversity of Transcriptional Regulatory Adaptation in E. coli.Molecular biology and evolution · 2024Article
- iModulonMiner and PyModulon: Software for unsupervised mining of gene expression compendia.PLoS computational biology · 2024Article
- Proteome allocation is linked to transcriptional regulation through a modularized transcriptome.Nature communications · 2024Article
- Laboratory evolution, transcriptomics, and modeling reveal mechanisms of paraquat tolerance.Cell reports · 2023Article
- Regulation of phosphate starvation-specific responses inMicrobiology (Reading, England) · 2023Review
- Proteome allocation is linked to transcriptional regulation through a modularized transcriptome.bioRxiv : the preprint server for biology · 2023Article
- Laboratory evolution of synthetic electron transport system variants reveals a larger metabolic respiratory system and its plasticity.Nature communications · 2022Article
- CRISPRi enables fast growth followed by stable aerobic pyruvate formation inEngineering in life sciences · 2022Article
- Antioxidant Mechanism ofFrontiers in microbiology · 2022Article
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Authors and funding
8 authors at 3 institutions in 4 countries.
Funding
Abstract
Pyruvate dehydrogenase complex (PDC) functions as the main determinant of the respiro-fermentative balance because it converts pyruvate to acetyl-coenzyme A (CoA), which then enters the TCA (tricarboxylic acid cycle). PDC is repressed by the pyruvate dehydrogenase complex regulator (PdhR) in Escherichia coli. The deletion of the pdhR gene compromises fitness in aerobic environments. We evolve the E. coli pdhR deletion strain to examine its achievable growth rate and the underlying adaptive strategies. We find that (1) optimal proteome allocation to PDC is critical in achieving optimal growth rate; (2) expression of PDC in evolved strains is reduced through mutations in the Shine-Dalgarno sequence; (3) rewiring of the TCA flux and increased reactive oxygen species (ROS) defense occur in the evolved strains; and (4) the evolved strains adapt to an efficient biomass yield. Together, these results show how adaptation can find alternative regulatory mechanisms for a key cellular process if the primary regulatory mode fails.
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