ArticleCell reports2021
High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo.
Article in Cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Designer indicators for two-photon recording of subthreshold voltage dynamics.Nature methods · 2026Article
- Voltage imaging as a window into neural computation.Neurophotonics · 2026Article
- Noise Sources and Strategies for Signal Quality Improvement in Biological Imaging: A Review Focused on Calcium and Cell Membrane Voltage Imaging.Biosensors · 2026Review
- Voltage imaging with periodic structured illumination.Biomedical optics express · 2026Article
- Real-time self-supervised denoising for high-speed fluorescence neural imaging.Nature communications · 2025Article
- Optical Recordings of Unitary Synaptic Connections Reveal High and Random Local Connectivity between CA3 Pyramidal Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Optical segmentation-based compressed readout of neuronal voltage dynamics.Nature communications · 2025Article
- Imaging neuronal voltage beyond the scattering limit.Nature methods · 2025Article
- Fluorescent sensors for intracellular signaling in the brain: imaging neurons, glia, and vascular cells.Neurophotonics · 2025Article
- Ultrafast optical imaging techniques for exploring rapid neuronal dynamics.Neurophotonics · 2025Review
- Optical constraints on two-photon voltage imaging.Neurophotonics · 2024Article
- Scanless two-photon voltage imaging.Nature communications · 2024Article
- Optical constraints on two-photon voltage imaging.bioRxiv : the preprint server for biology · 2024Article
- Widefield imaging of rapid pan-cortical voltage dynamics with an indicator evolved for one-photon microscopy.Nature communications · 2023Article
- Statistically unbiased prediction enables accurate denoising of voltage imaging data.Nature methods · 2023Article
- FIOLA: an accelerated pipeline for fluorescence imaging online analysis.Nature methods · 2023Article
- Scanless two-photon voltage imaging.Research square · 2023Article
- Temporal dynamics of Na/K pump mediated memory traces: insights from conductance-based models ofFrontiers in neuroscience · 2023Article
- Multiregion neuronal activity: the forest and the trees.Nature reviews. Neuroscience · 2022Review
- Review of data processing of functional optical microscopy for neuroscience.Neurophotonics · 2022Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The ability to probe the membrane potential of multiple genetically defined neurons simultaneously would have a profound impact on neuroscience research. Genetically encoded voltage indicators are a promising tool for this purpose, and recent developments have achieved a high signal-to-noise ratio in vivo with 1-photon fluorescence imaging. However, these recordings exhibit several sources of noise and signal extraction remains a challenge. We present an improved signal extraction pipeline, spike-guided penalized matrix decomposition-nonnegative matrix factorization (SGPMD-NMF), which resolves supra- and subthreshold voltages in vivo. The method incorporates biophysical and optical constraints. We validate the pipeline with simultaneous patch-clamp and optical recordings from mouse layer 1 in vivo and with simulated and composite datasets with realistic noise. We demonstrate applications to mouse hippocampus expressing paQuasAr3-s or SomArchon1, mouse cortex expressing SomArchon1 or Voltron, and zebrafish spines expressing zArchon1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.