Evidence map›Paper›PMID 33826490›Full record

ArticleMicrobial genomics2021

Application of a strain-level shotgun metagenomics approach on food samples: resolution of the source of a

Florence E Buytaers, Assia Saltykova, Wesley Mattheus, Bavo Verhaegen, Nancy H C Roosens, Kevin Vanneste, Valeska Laisnez, Naïma Hammami, Brigitte Pochet, Vera Cantaert and 3 more

Open access · goldAbstract readEvaluation Study
In one paragraph

Article in Microbial genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
5.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 29 citations in OpenAlex.

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  11. Review
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  13. mStrain: strain-level identification ofBioinformatics advances · 2023
    Article
  14. bettercallsal: better calling ofFrontiers in microbiology · 2023
    Article
  15. Article
  16. Article
  17. Genomic Surveillance of Foodborne Pathogens: Advances and Obstacles.Journal of public health management and practice : JPHMP
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Florence E BuytaersTransversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.
Assia SaltykovaTransversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.
Wesley MattheusNational Reference Center for Salmonella and Shigella spp., Sciensano, Brussels, Belgium.
Bavo VerhaegenNational Reference Laboratory for Salmonella and Food-Borne Infections, Food-Borne Pathogens, Sciensano, Brussels, Belgium.
Nancy H C RoosensTransversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.
Kevin VannesteTransversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.
Valeska LaisnezAgentschap Zorg en Gezondheid, Brussels, Belgium.
Naïma HammamiAgentschap Zorg en Gezondheid, Brussels, Belgium.
Brigitte PochetFederal Agency for the Security of the Food Chain, Brussels, Belgium.
Vera CantaertFederal Agency for the Security of the Food Chain, Brussels, Belgium.
Kathleen MarchalDepartment of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
Sarah DenayerNational Reference Laboratory for Salmonella and Food-Borne Infections, Food-Borne Pathogens, Sciensano, Brussels, Belgium.
Sigrid C J De KeersmaeckerTransversal Activities in Applied Genomics, Sciensano, Brussels, Belgium.
Sciensano (Belgium) · BEGhent University · BEFederal Agency for Food Chain Safety · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Food-borne outbreak investigation currently relies on the time-consuming and challenging bacterial isolation from food, to be able to link food-derived strains to more easily obtained isolates from infected people. When no food isolate can be obtained, the source of the outbreak cannot be unambiguously determined. Shotgun metagenomics approaches applied to the food samples could circumvent this need for isolation from the suspected source, but require downstream strain-level data analysis to be able to accurately link to the human isolate. Until now, this approach has not yet been applied outside research settings to analyse real food-borne outbreak samples. In September 2019, a

Indexed as

Bacterial Typing TechniquesBelgiumDisease OutbreaksHumansMetagenomicsPhylogenySalmonellaSalmonella Food Poisoningfood surveillancemetagenomicsoutbreakSalmonellaSNP analysisstrain-level

Identifiers

PMID33826490
PMCPMC8208685
OpenAlexW3150669222

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.