Evidence map›Paper›PMID 33823914›Full record

ArticleParasites & vectors2021

Vaccination against the brown stomach worm, Teladorsagia circumcincta, followed by parasite challenge, induces inconsistent modifications in gut microbiota composition of lambs.

James Rooney, Alba Cortés, Riccardo Scotti, Daniel R G Price, Yvonne Bartley, Karen Fairlie-Clarke, Tom N McNeilly, Alasdair J Nisbet, Cinzia Cantacessi

Open access · goldAbstract readClinical Trial, Veterinary
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

James RooneyDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Alba Cortés *Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Riccardo Scotti *Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.
Daniel R G PriceVaccines and Diagnostics Department, Moredun Research Institute, Edinburgh, UK.
Yvonne BartleyVaccines and Diagnostics Department, Moredun Research Institute, Edinburgh, UK.
Karen Fairlie-ClarkeVaccines and Diagnostics Department, Moredun Research Institute, Edinburgh, UK.
Tom N McNeillyDisease Control Department, Moredun Research Institute, Edinburgh, UK.
Alasdair J NisbetVaccines and Diagnostics Department, Moredun Research Institute, Edinburgh, UK.
Cinzia CantacessiDepartment of Veterinary Medicine, University of Cambridge, Cambridge, UK. cc779@cam.ac.uk.ORCID http://orcid.org/0000-0001-6863-2950
Moredun Research Institute · GBUniversity of Cambridge · GBUniversitat de València · ES

Funding

Biotechnology and Biological Sciences Research Council BB/L027186/1Horizon 2020 () 635408
6 · The paper itself

Abstract

backgroundGrowing evidence points towards a role of gastrointestinal (GI) helminth parasites of ruminants in modifying the composition of the host gut flora, with likely repercussions on the pathophysiology of worm infection and disease, and on animal growth and productivity. However, a thorough understanding of the mechanisms governing helminth-microbiota interactions and of their impact on host health and welfare relies on reproducibility and replicability of findings. To this aim, in this study, we analysed quantitative and qualitative fluctuations in the faecal microbiota composition of lambs vaccinated against, and experimentally infected with, the parasitic GI nematode Teladorsagia circumcincta over the course of two separate trials performed over two consecutive years.

methodsTwo trials were conducted under similar experimental conditions in 2017 and 2018, respectively. In each trial, lambs were randomly assigned to one of the following experimental groups: (i) vaccinated/infected, (ii) unvaccinated/infected and (iii) unvaccinated/uninfected. Faecal samples collected from individual animals were subjected to DNA extraction followed by high-throughput sequencing of the V3-V4 region of the bacterial 16S rRNA gene and bioinformatics and biostatistical analyses of sequence data.

resultsSubstantial differences in the populations of bacteria affected by immunisation against and infection by T. circumcincta were detected when comparing data from the two trials. Nevertheless, the abundance of Prevotella spp. was significantly linked to helminth infection in both trials.

conclusionsDespite the largely conflicting findings between the two trials, our data revealed that selected gut microbial populations are consistently affected by T. circumcincta infection and/or vaccination. Nevertheless, our study calls for caution when interpreting data generated from in vivo helminth-microbiome interaction studies that may be influenced by several intrinsic and extrinsic host-, parasite- and environment-related factors.

Indexed as

AnimalsAntibodies, HelminthBacteriaFecesGastrointestinal MicrobiomeIntestinal Diseases, ParasiticOstertagiaParasite Egg CountReproducibility of ResultsRNA, Ribosomal, 16SSheepSheep DiseasesTrichostrongyloidiasisVaccinationAntibodies, HelminthRNA, Ribosomal, 16SGastrointestinal helminthMicrobiomePrevotella spp.RuminantTeladorsagia circumcinctaVaccine

Identifiers

PMID33823914
PMCPMC8025363
OpenAlexW3145486538

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.