ReviewThe Plant cell2021
CRISPR screens in plants: approaches, guidelines, and future prospects.
Review in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 97 citations in OpenAlex.
- Accelerating Resistance Breeding: Emerging Methods to Identify and Validate Plant Immunity Genes.Plants (Basel, Switzerland) · 2026Review
- A CRISPR-Cas9 library to target putative redundant gene sets facilitates their functional exploration in grain development in rice.BMC plant biology · 2025Article
- Review
- Impact of Temperature Stresses on Wheat Quality: A Focus on Starch and Protein Composition.Foods (Basel, Switzerland) · 2025Review
- CRISPR-Cas applications in agriculture and plant research.Nature reviews. Molecular cell biology · 2025Review
- Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics.Plants (Basel, Switzerland) · 2025Review
- A comprehensive all-in-one CRISPR toolbox for large-scale screens in plants.The Plant cell · 2025Article
- Jan and mini-Jan, a model system for potato functional genomics.Plant biotechnology journal · 2025Article
- Maize COMPACT PLANT 3 regulates plant architecture and facilitates high-density planting.The Plant cell · 2025Article
- Construction of a Cas9-targeted mutagenesis mini-library in the upland cotton genetic standard line (TM-1).Plant cell reports · 2025Article
- Small signaling peptides define leaf longevity.Frontiers in plant science · 2025Article
- A micropeptide regulates seed desiccation.Frontiers in plant science · 2025Article
- Malicious DNS detection by combining improved transformer and CNN.Scientific reports · 2024Article
- CRISPR-Cas9-mediated construction of a cotton CDPK mutant library for identification of insect-resistance genes.Plant communications · 2024Article
- A CRISPR/Cas9-mediated mutant library of seed-preferred genes in rice.Plant biotechnology journal · 2024Article
- How Helpful May Be a CRISPR/Cas-Based System for Food Traceability?Foods (Basel, Switzerland) · 2024Review
- Review
- Review
- Breeding of ornamental orchids with focus on Phalaenopsis: current approaches, tools, and challenges for this century.Heredity · 2024Review
- Pooled effector library screening in protoplasts rapidly identifies novel Avr genes.Nature plants · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clustered regularly interspaced short palindromic repeat (CRISPR)-associated systems have revolutionized genome engineering by facilitating a wide range of targeted DNA perturbations. These systems have resulted in the development of powerful new screens to test gene functions at the genomic scale. While there is tremendous potential to map and interrogate gene regulatory networks at unprecedented speed and scale using CRISPR screens, their implementation in plants remains in its infancy. Here we discuss the general concepts, tools, and workflows for establishing CRISPR screens in plants and analyze the handful of recent reports describing the use of this strategy to generate mutant knockout collections or to diversify DNA sequences. In addition, we provide insight into how to design CRISPR knockout screens in plants given the current challenges and limitations and examine multiple design options. Finally, we discuss the unique multiplexing capabilities of CRISPR screens to investigate redundant gene functions in highly duplicated plant genomes. Combinatorial mutant screens have the potential to routinely generate higher-order mutant collections and facilitate the characterization of gene networks. By integrating this approach with the numerous genomic profiles that have been generated over the past two decades, the implementation of CRISPR screens offers new opportunities to analyze plant genomes at deeper resolution and will lead to great advances in functional and synthetic biology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.