Evidence map›Paper›PMID 33821317›Full record

ArticleActa neurochirurgica2021

Exosomes secreted from sonic hedgehog-modified bone mesenchymal stem cells facilitate the repair of rat spinal cord injuries.

Yijia Jia, Tingsheng Lu, Qiling Chen, Xingwei Pu, Linsong Ji, Jianwen Yang, Chunshan Luo

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta neurochirurgica, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 3 pooled it
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 3 syntheses or guidelines pooled it, 30 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Exosomes combined with biomaterials in the treatment of spinal cord injury.Frontiers in bioengineering and biotechnology · 2023
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yijia JiaDepartment of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
Tingsheng LuDepartment of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
Qiling ChenDepartment of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
Xingwei PuDepartment of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
Linsong JiDepartment of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
Jianwen YangDepartment of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong South Street, Nanming District, Guiyang, 550002, Guizhou Province, China.
Chunshan LuoDepartment of Spine Surgery, Guizhou Province Osteological Hospital, 123 Shachong South Street, Nanming District, Guiyang, 550002, Guizhou Province, China. jiayijia2192@126.com.
Guizhou Provincial People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSpinal cord injuries (SCIs) can cause a loss of neurons and associated sensory and motor functionality below the injured site. No approaches to treating SCIs in humans have been developed to date. Exosomes are extracellular vesicles that hold promise as a potential therapeutic modality when treating such injuries. The present study was thus designed to determine whether sonic hedgehog (Shh)-overexpressing bone mesenchymal stem cell (BMSC)-derived exosomes were protective in the context of SCIs.

methodsExosomes were extracted from control or Shh lentivirus-transduced BMSCs, yielding respective BMSC-Exo and BMSC-Shh-Exo preparations which were intravenously injected into SCI model rats. Shh expression in spinal cord tissues in these animals was then assessed via immunohistochemical staining, while Basso-Beattie-Bresnahan (BBB) scores were utilized to measure high limb motor function. Neuronal damage and regeneration within the spinal cord were additionally evaluated via terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), Nissl, hematoxylin and eosin, and immunofluorescent staining.

resultsBoth BMSC-Exo and BMSC-Shh-Exo preparations significantly increased Shh expression in the spinal cord of SCI model rats and improved BBB scores in these treated animals, while also increasing the frequencies of Nissl- and NeuN-positive neurons are reducing the numbers of apoptotic and GFAP-positive neurons. While both treatments yielded some degree of benefit to treated animals relative to untreated controls, BMSC-Shh-Exos were more beneficial than were control BMSC-Exos.

conclusionsShh-overexpressing BMSC-derived exosomes represent an effective treatment that can facilitate SCI repair in rats.

Indexed as

ExosomesMesenchymal Stem CellsMesenchymal Stem Cell TransplantationSpinal Cord InjuriesAnimalsHedgehog ProteinsMaleRatsRats, Sprague-DawleyRecovery of FunctionSpinal CordHedgehog ProteinsBMSCsExosomesSonic hedgehogSpinal cord injury

Identifiers

PMID33821317
PMCPMC8270837
OpenAlexW3148557228

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.