ArticleNature biomedical engineering2021
Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor.
Article in Nature biomedical engineering, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Graphene Nanosensor for the Detection of Small Organic Compounds Using an Insect Olfactory Receptor.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- The Application of Flexible Graphene Field-Effect Transistor Sensors in Multidimensional Biosensing and Precision Medicine.Materials (Basel, Switzerland) · 2026Review
- Biophysical characterization ofSensors & diagnostics · 2026Article
- CRISPR Diagnostics, in Your Pocket.Journal of medical Internet research · 2026Article
- A transistor-based point-of-care assay with lipid-capped sensory interface for clinical profiling of cardiovascular diseases.National science review · 2026Article
- Nucleic Acid-Based Field-Effect Transistor Biosensors.Biosensors · 2026Review
- Advances in Double-Stranded DNA Targeting Technologies.Exploration (Beijing, China) · 2026Review
- CRISPR-on-Chip for Point-of-Care Diagnostics.ACS nano · 2026Review
- Graphene Growth via Plasma-Enhanced Hybrid Physical-Chemical Vapor Deposition: A Catalyst-Free Approach.ACS omega · 2025Article
- Convergence of nanotechnology and CRISPR-based diagnostics.Nature nanotechnology · 2025Review
- Recent advances in CRISPR-based single-nucleotide fidelity diagnostics.Communications medicine · 2025Review
- Recent Advances in Xenes Based FET for Biosensing Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Advances in 2D materials for wearable biomonitoring.Materials science & engineering. R, Reports : a review journal · 2025Article
- Nanotechnology-leveraged CRISPR/Cas systems: icebreaking in trace cancer-related nucleic acids biosensing.Molecular cancer · 2025Review
- A portable and cost-effective system for electronic nucleic acid mass measurement.Scientific reports · 2025Article
- Coupling CRISPR-Cas and a personal glucose meter with an enzymatic reporter for portable detection of human papillomavirus in biological samples.Theranostics · 2025Article
- Real-Time Monitoring in Biomanufacturing with Graphene Field-Effect Transistor Sensors: Detection of pH, Glucose, and Antibodies.GEN biotechnology · 2024Article
- Localization and discrimination of GG mismatch in duplex DNA by synthetic ligand-enhanced protein nanopore analysis.Nucleic acids research · 2024Article
- CRISPR-Cas Systems Associated with Electrolyte-Gated Graphene-Based Transistors: How They Work and How to Combine Them.Biosensors · 2024Review
- CRISPR for companion diagnostics in low-resource settings.Lab on a chip · 2024Review
Corrections and comments
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Authors and funding
21 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simple and fast methods for the detection of target genes with single-nucleotide specificity could open up genetic research and diagnostics beyond laboratory settings. We recently reported a biosensor for the electronic detection of unamplified target genes using liquid-gated graphene field-effect transistors employing an RNA-guided catalytically deactivated CRISPR-associated protein 9 (Cas9) anchored to a graphene monolayer. Here, using unamplified genomic samples from patients and by measuring multiple types of electrical response, we show that the biosensors can discriminate within one hour between wild-type and homozygous mutant alleles differing by a single nucleotide. We also show that biosensors using a guide RNA-Cas9 orthologue complex targeting genes within the protospacer-adjacent motif discriminated between homozygous and heterozygous DNA samples from patients with sickle cell disease, and that the biosensors can also be used to rapidly screen for guide RNA-Cas9 complexes that maximize gene-targeting efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.