ArticleVeterinary medicine and science2021
Berberine regulates lipid metabolism via miR-192 in porcine oocytes matured in vitro.
Article in Veterinary medicine and science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 7 citations in OpenAlex.
- Targeting lipid droplets and lipid droplet-associated proteins: a new perspective on natural compounds against metabolic diseases.Chinese medicine · 2024Review
- Effects of β-Nicotinamide Mononucleotide, Berberine, and Cordycepin on Lipid Droplet Content and Developmental Ability of Vitrified Bovine Oocytes.Antioxidants (Basel, Switzerland) · 2023Article
- Advances of berberine against metabolic syndrome-associated kidney disease: Regarding effect and mechanism.Frontiers in pharmacology · 2023Review
- Article
- Protective role of trametenolic acid B against sevoflurane-induced cognitive impairments by its different regulatory modalities of mir-329-3p in neurons and microglia.Molecular medicine (Cambridge, Mass.) · 2022Article
- Berberine alleviates LPS-induced apoptosis, oxidation, and skewed lineages during mouse preimplantation development†.Biology of reproduction · 2022Article
- Berberine regulates lipid metabolism via miR-192 in porcine oocytes matured in vitro.Veterinary medicine and science · 2021Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe berberine (Ber) is an isoquinoline alkaloid compound extracted from Rhizoma coptidis and has the effect that reduces adipose. MicroRNA-192 (miR-192) is related to fat metabolism. However, the relevant mechanism of berberine on lipid metabolism during in vitro maturation (IVM) of porcine oocytes remains unclear.
objectivesIn this study, we investigated the molecular mechanism by which berberine promotes the IVM and lipid metabolism of porcine oocytes via miR-192.
methodsBer was added to IVM medium of porcine oocytes. MiR-192 agomir, miR-192 antagomir and negative control fragment were microinjected into the cytoplasm of oocytes without Ber. Rates of oocyte IVM and embryonic development in each group were observed. The content of lipid droplets in IVM oocytes in each group was analyzed by Nile red staining. Expression levels of miR-192 and FABP3, SREBF1 and PPARG, were detected by qPCR and western blotting. The target genes of miR-192 were determined by luciferase reporter assays. RESULTS AND
conclusionsWe found that Ber significantly increased the rate of oocytes IVM and blastocyst development, and decreased the area and numbers of lipid droplets in IVM oocytes. Ber significantly increased the expression of miR-192 in IVM oocytes, and significantly decreased the expression of SREBF1 and PPARG, which were target genes of miR-192. This study indicates that Ber promotes lipid metabolism in porcine oocytes by activating the expression of miR-192 and down-regulating SREBF1 and PPARG, thus, improving IVM of porcine oocytes.
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