Evidence map›Paper›PMID 33817392›Full record

ArticleJournal of veterinary research2021

Characterisation of a New Molecule Based on Two E2 Sequences from Bovine Viral Diarrhoea-mucosal Disease Virus Fused To the Human Immunoglobulin Fc Fragment.

Alaín González Pose, Raquel Montesino Seguí, Rafael Maura Pérez, Florence Hugues Salazar, Ignacio Cabezas Ávila, Claudia Altamirano Gómez, Oliberto Sánchez Ramos, Jorge Roberto Toledo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Alaín González PoseBiotechnology and Biopharmaceutical Laboratory, Pathophysiology Department, School of Biological Sciences, University of Concepcion, Victor Lamas 1290, P.O. Box 160-C, 4030000 Concepcion, Chile.
Raquel Montesino SeguíBiotechnology and Biopharmaceutical Laboratory, Pathophysiology Department, School of Biological Sciences, University of Concepcion, Victor Lamas 1290, P.O. Box 160-C, 4030000 Concepcion, Chile.
Rafael Maura PérezBiotechnology and Biopharmaceutical Laboratory, Pathophysiology Department, School of Biological Sciences, University of Concepcion, Victor Lamas 1290, P.O. Box 160-C, 4030000 Concepcion, Chile.
Florence Hugues SalazarBiotechnology and Biopharmaceutical Laboratory, Pathophysiology Department, School of Biological Sciences, University of Concepcion, Victor Lamas 1290, P.O. Box 160-C, 4030000 Concepcion, Chile.
Ignacio Cabezas ÁvilaLarge Animal Clinic, Clinical Sciences Department, School of Veterinary Sciences, University of Concepcion, Vicente Mendez 595, P.O. Box 537, 3780000 Chillan, Chile.
Claudia Altamirano GómezSchool of Biochemical Engineering, Pontifical Catholic University of Valparaiso, Valparaiso, Chile CREAS CONICYT-Regional, GORE Valparaiso, Universidad Avenue 300, Placilla, Curauma, 2340000 Valparaiso, Chile.
Oliberto Sánchez RamosRecombinant Biopharmaceuticals Laboratory, Pharmacology Department, School of Biological Sciences, University of Concepcion, Victor Lamas 1290, P.O. Box 160-C, 4030000 Concepcion, Chile.
Jorge Roberto ToledoBiotechnology and Biopharmaceutical Laboratory, Pathophysiology Department, School of Biological Sciences, University of Concepcion, Victor Lamas 1290, P.O. Box 160-C, 4030000 Concepcion, Chile.
University of Concepción · CLUniversity of Valparaíso · CL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionProper conformational arrangement of the E2 molecules of bovine viral diarrhoea-mucosal disease virus (BVD-MDV) is crucial to obtain an effective recombinant vaccine candidate against the disease. In this study, we characterised a new molecule composed of two distinct sequences of the E2 glycoprotein of BVD-MDV and the Fc fragment of human immunoglobulin (BVDE2Fc). MATERIALS AND

methodsThe chimaeric protein was expressed in mammalian cell lines of different species by adenoviral transduction and purified by immobilised metal-affinity chromatography. The N-glycans were profiled by HPLC, and the BVDE2Fc immunogenicity was assessed in male mice. The antigen-antibody reactions were evaluated by ELISA.

resultsThe MDBK cell line was selected from among five for the final production of BVDE2Fc. After purification to over 90%, the N-glycan profile showed neutral and complex oligosaccharides. The mouse immunisation induced a strong humoral response, which produced antibodies able to attach to conformational epitopes on E2 molecules, while the Fc fragment barely contributed to the immune response. Additionally, BVDE2Fc attached to antibodies from bovine sera positive to distinct BVD-MDV subtypes, whereas the loss of BVDE2Fc structure during the deglycosylation process considerably diminished those interactions.

conclusionThese results demonstrate that the structure of E2 molecules arranged in tandem and attached to an Fc fragment could represent a viable design for future vaccine candidates against BVD-MD.

Indexed as

adenoviral vectorbovine viral diarrhoea virusE2 glycoproteinexpression systemimmune response

Identifiers

PMID33817392
PMCPMC8009577
OpenAlexW3134007088

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.