ArticleFrontiers in veterinary science2021
The Isolation and Replication of African Swine Fever Virus in Primary Renal-Derived Swine Macrophages.
Article in Frontiers in veterinary science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 14 citations in OpenAlex.
- African swine fever: A highly fatal disease that is spreading globally.Open veterinary journal · 2025Review
- Article
- African Local Pig Genetic Resources in the Context of Climate Change Adaptation.Animals : an open access journal from MDPI · 2024Review
- African Swine Fever Diagnosis in Africa: Challenges and Opportunities.Pathogens (Basel, Switzerland) · 2024Review
- Population Structure and Genomic Characterisation of the Ashanti Dwarf Pig of Ghana.Animals : an open access journal from MDPI · 2024Article
- CA-CAS-01-A: A Permissive Cell Line for Isolation and Live Attenuated Vaccine Development Against African Swine Fever Virus.Journal of microbiology (Seoul, Korea) · 2024Article
- Macrophages Characterization in an Injured Bone Tissue.Biomedicines · 2022Article
- Cell Lines for the Development of African Swine Fever Virus Vaccine Candidates: An Update.Vaccines · 2022Review
- Chronological expression and distribution of African swine fever virus p30 and p72 proteins in experimentally infected pigs.Scientific reports · 2022Article
- Structural Design and Assessing of Recombinantly Expressed African Swine Fever Virus p72 Trimer inFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
African swine fever virus (ASFV) causes hemorrhagic disease in domestic pigs by replicating mainly in monocyte/macrophage lineages. Various primary cells including pulmonary alveolar macrophages have been used for the propagation of ASFV on this account. However, ethical constraints and consistency problems exist as it is necessary to harvest same phenotype of primary cells in order to continue a study. We suggested renal-derived swine macrophages as a novel primary cell candidate to address these issues. These primary cells proved to be permissive to both cell adapted ASFV and a wild-type ASFV. Compared to the commercial cell line MA-104, the renal-derived macrophages were more suitable to isolate the field virus. The consistent molecular characteristics of the renal-derived macrophages were demonstrated by immunocytochemistry with antibodies against macrophage cell surface markers including CD163, CD172a, and Iba-1. Viral protein p30 and p72 expression in ASFV infected macrophages was confirmed by immunocytochemistry by use of specific monoclonal antibodies. We observed increase of cell-free viral DNA and infectious virus titer in infected cell supernatant in successive days-post-infection. These results demonstrated that primary renal-derived swine macrophages are useful for ASFV isolation and propagation in terms of cell phenotypes, susceptibility to the virus, and virus production.
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