ArticleAnalytical methods : advancing methods and applications2019
Mass Spectrometry Imaging (MSI) of Fresh Bones using Infrared Matrix-Assisted Laser Desorption Electrospray Ionization (IR-MALDESI).
Article in Analytical methods : advancing methods and applications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Comprehensive High-Spatial-Resolution Imaging Metabolomics Workflow for Heterogeneous Tissues.Analytical chemistry · 2025Article
- In situ metabolomic analysis of osteonecrosis of the femoral head (ONFH) using MALDI MSI.Analytical and bioanalytical chemistry · 2024Article
- Optimizing neurotransmitter pathway detection by IR-MALDESI-MSI in mouse brain.Analytical and bioanalytical chemistry · 2024Article
- Mass Spectrometry Reveals Molecular Effects of Citrulline Supplementation during Bone Fracture Healing in a Rat Model.Journal of the American Society for Mass Spectrometry · 2024Article
- Recent Developments and Application of Mass Spectrometry Imaging in N-Glycosylation Studies: An Overview.Mass spectrometry (Tokyo, Japan) · 2024Review
- The development and application of matrix assisted laser desorption electrospray ionization: The teenage years.Mass spectrometry reviews · 2023Review
- Multimodal Mass Spectrometry Imaging of Rat Brain Using IR-MALDESI and NanoPOTS-LC-MS/MS.Journal of proteome research · 2022Article
- Mass Spectrometry Imaging ofJournal of proteome research · 2022Article
- Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis.Metabolites · 2021Review
- Spatially resolved metabolomic characterization of muscle invasive bladder cancer by mass spectrometry imaging.Metabolomics : Official journal of the Metabolomic Society · 2021Article
- Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.Analytical and bioanalytical chemistry · 2021Article
- A Versatile Platform for Mass Spectrometry Imaging of Arbitrary Spatial Patterns.Journal of the American Society for Mass Spectrometry · 2020Article
- Lipidomic profiling of single mammalian cells by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI).Analytical and bioanalytical chemistry · 2020Article
- Coupling IR-MALDESI with Drift Tube Ion Mobility-Mass Spectrometry for High-Throughput Screening and Imaging Applications.Journal of the American Society for Mass Spectrometry · 2020Article
- Integrating Remote Sensing and Metabolomics to Assess Synergistic Effects of Phosphate Deficiency, Drought, and AMF Symbiosis in Soybean.Physiologia plantarumArticle
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Authors and funding
4 authors.
Funding
Abstract
We report an effective strategy for direct analysis and two-dimensional (2D) matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging (MSI) of mouse bones that underwent no chemical treatments prior to analysis. To unravel the chemistry in bones under near-physiological conditions, we cut a flash-frozen bone in half longitudinally, placed it in a mold facing flat side down, and poured Plaster of Paris on top of and around the bone. After Plaster of Paris had set, the bone with embedding material was removed from the mold, and placed on the IR-MALDESI imaging stage. Plaster of Paris acted as a fixture to keep every spot on the sample surface the same distance away from the laser focus. To demonstrate the feasibility of IR-MALDESI MSI for analyses of unmodified bones, we imaged bones derived from healthy and stroke-affected mice and generated ion heatmaps showing the spatial distribution of putatively annotated features.
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