ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2021
Sticky problems: extraction of nucleic acids from molluscs.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- DNA Extraction Optimisation for Minute Land Snails ofEcology and evolution · 2026Article
- Genetic differentiation of the supralittoral gastropod Tectarius striatus (North Atlantic Archipelagos) and development of new microsatellite resources.BMC ecology and evolution · 2026Article
- Phylogeography and Population Structure of the Invasive Land SnailInternational journal of molecular sciences · 2026Article
- Chromosome-level genome provides new insights into the fatty acid biosynthesis and metabolism of the sea urchin Strongylocentrotus intermedius.BMC genomics · 2026Article
- The complete mitogenome of Tylomelania sp. 'Poso A' (Caenogastropoda: Pachychilidae) from Lake Poso assembled from long-read sequencing data.Molecular biology reports · 2026Article
- From Permits to Samples: Addressing Key Challenges for High-Quality Reference Genome Generation in Europe.Molecular ecology resources · 2026Review
- Annotated genome of the Atlantic dog whelk, Nucella lapillus.G3 (Bethesda, Md.) · 2025Article
- Re-Evaluation of a Hyperendemic Focus of Metastrongyloid Lungworm Infections in Gastropod Intermediate Hosts in Southern Germany.Pathogens (Basel, Switzerland) · 2025Article
- CRISPR/Cas9 Knockout of Shell Matrix Protein 1 in the Slipper-Snail Crepidula atrasolea.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2025Article
- Genomic, transcriptomic and epigenomic signatures of ageing and cold adaptation in the Antarctic clamOpen biology · 2025Article
- Establishing genome sequencing and assembly for non-model and emerging model organisms: a brief guide.Frontiers in zoology · 2025Review
- Combination of Sample Preservation Approaches and DNA Extraction Methods for Long-Read Sequencing of Nudibranchs' Genomes.Ecology and evolution · 2025Article
- A RAPID DIAGNOSTIC PCR ASSAY FOR THE DETECTION OF SCHISTOSOMA MANSONI IN THEIR SNAIL VECTORS.The Journal of parasitology · 2024Article
- Of Clams and Clades: Genetic Diversity and Connectivity of Small Giant Clams (Ecology and evolution · 2024Article
- The development of multiplex PCR assays for the rapid identification of multiple Saccostrea species, and their practical applications in restoration and aquaculture.BMC ecology and evolution · 2024Article
- Development, validation, and pilot application of a high throughput molecular xenomonitoring assay to detectCurrent research in parasitology & vector-borne diseases · 2024Article
- Extraction of genomic DNA for sequencing from snailJournal of genetics · 2024Article
- Fine mapping of the Cepaea nemoralis shell colour and mid-banded loci using a high-density linkage map.Heredity · 2023Article
- Functional genomics in Spiralia.Briefings in functional genomics · 2023Article
- Amplicon-Based High-Throughput Sequencing Method for Genotypic Characterization of Norovirus in Oysters.Applied and environmental microbiology · 2023Article
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Abstract
Traditional molecular methods and omics-techniques across molluscan taxonomy increasingly inform biology of Mollusca. Recovery of DNA and RNA for such studies is challenged by common biological properties of the highly diverse molluscs. Molluscan biomineralization, adhesive structures and mucus involve polyphenolic proteins and mucopolysaccharides that hinder DNA extraction or copurify to inhibit enzyme-catalysed molecular procedures. DNA extraction methods that employ the detergent hexadecyltrimethylammoniumbromide (CTAB) to remove these contaminants importantly facilitate molecular-level study of molluscs. Molluscan pigments may stain DNA samples and interfere with spectrophotometry, necessitating gel electrophoresis or fluorometry for accurate quantification. RNA can reliably be extracted but the 'hidden break' in 28S rRNA of molluscs (like most protostomes) causes 18S and 28S rRNA fragments to co-migrate electrophoretically. This challenges the standard quality control based on the ratio of 18S and 28S rRNA, developed for deuterostome animals. High-AT content in molluscan rRNA prevents the effective purification of polyadenylated mRNA. Awareness of these matters aids the continuous expansion of molecular malacology, enabling work also with museum specimens and next-generation sequencing, with the latter imposing unprecedented demands on DNA quality. Alternative methods to extract nucleic acids from molluscs are available from literature and, importantly, from communications with others who study the molecular biology of molluscs. This article is part of the Theo Murphy meeting issue 'Molluscan genomics: broad insights and future directions for a neglected phylum'.
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