Evidence map›Paper›PMID 33813702›Full record

ArticleEnvironmental science and pollution research international2021

Rapid and sensitive detection of Karlodinium veneficum by a novel double-nick rolling circle amplification.

Fuguo Liu, Chunyun Zhang, Huiwen Zheng, Yuchen Yang, Diya Zang, Yuanyuan Wang, Guofu Chen

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In one paragraph

Article in Environmental science and pollution research international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 13 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Fuguo LiuSchool of Marine Science and Technology, Harbin Institute of Technology (Weihai), Harbin Institute of Technology (Weihai), Wenhua West Road, 2#,, Weihai, 264209, Shandong Province, People's Republic of China.
Chunyun ZhangSchool of Marine Science and Technology, Harbin Institute of Technology (Weihai), Harbin Institute of Technology (Weihai), Wenhua West Road, 2#,, Weihai, 264209, Shandong Province, People's Republic of China. zhangchunyun@hitwh.edu.cn.
Huiwen ZhengSchool of Marine Science and Technology, Harbin Institute of Technology (Weihai), Harbin Institute of Technology (Weihai), Wenhua West Road, 2#,, Weihai, 264209, Shandong Province, People's Republic of China.
Yuchen YangSchool of Marine Science and Technology, Harbin Institute of Technology (Weihai), Harbin Institute of Technology (Weihai), Wenhua West Road, 2#,, Weihai, 264209, Shandong Province, People's Republic of China.
Diya ZangSchool of Marine Science and Technology, Harbin Institute of Technology (Weihai), Harbin Institute of Technology (Weihai), Wenhua West Road, 2#,, Weihai, 264209, Shandong Province, People's Republic of China.
Yuanyuan WangSchool of Marine Science and Technology, Harbin Institute of Technology (Weihai), Harbin Institute of Technology (Weihai), Wenhua West Road, 2#,, Weihai, 264209, Shandong Province, People's Republic of China.
Guofu ChenSchool of Marine Science and Technology, Harbin Institute of Technology (Weihai), Harbin Institute of Technology (Weihai), Wenhua West Road, 2#,, Weihai, 264209, Shandong Province, People's Republic of China. chenguofu@hitwh.edu.cn.
Harbin Institute of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Harmful algal blooms caused by Karlodinium veneficum recently occurred with high incidence, posing a serious threat to the marine ecological environment, public health, and mariculture. It is therefore rather vital to establish a method for rapid detection of K. veneficum. In this study, the D1-D2 region of the large subunit rDNA (LSU rDNA D1-D2) of K. veneficum was cloned and sequenced to design the specific probes and primers. A novel method referred to as double-nick rolling circle amplification (dn-RCA) based on the designed probes and primers was initially established. The optimal reaction conditions for dn-RCA were as follows: probe concentration, 200 pM; ligation temperature, 57 °C; ligation time, 50 min; amplification temperature, 60 °C; and amplification time, 60 min. Furthermore, lateral flow dipstick (LFD) was employed instead of agarose gel electrophoresis to analyze dn-RCA products, which can simplify the detection procedure and reduce the operation time. The sensitivity of dn-RCA-LFD was tested with the genomic DNA, the recombinant plasmid containing the inserted LSU rDNA D1-D2, and the DNA crude extract of K. veneficum. The results showed that the sensitivity of dn-RCA-LFD was 10 times higher than that of conventional PCR; the detection limit of dn-RCA-LFD was 1.1 × 10

Indexed as

DinoflagellidaNucleic Acid Amplification TechniquesDNA PrimersHarmful Algal BloomPolymerase Chain ReactionSensitivity and SpecificityDNA PrimersDetectionDouble-nick rolling circle amplificationKarlodinium veneficumLarge submit rDNALateral flow dipstick

Identifiers

PMID33813702
OpenAlexW3147785568

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.