ArticleFish physiology and biochemistry2021
Both TGF-β1 and Smad4 regulate type I collagen expression in the muscle of grass carp, Ctenopharyngodon idella.
Article in Fish physiology and biochemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Effects of Mulberry Leaf Extract on the Growth Performance, Organ Indices, Flesh Quality, Collagen Metabolism, and Myofiber Development of Grass Carp (International journal of molecular sciences · 2026Article
- Microbiome-Directed Bioactive Strategies in Skin Aging: Mechanistic Insights and Precision Nanocarrier Delivery Approaches.Molecules (Basel, Switzerland) · 2026Review
- Pten-Mediated Antioxidant Response Alleviates Hydrogen Peroxide-Induced Oxidative Damage in Tilapia Muscle.Antioxidants (Basel, Switzerland) · 2026Article
- Glutamine Promotes Collagen Deposition via TGF-β/Smads Pathway in Swim Bladder of Chu's Croaker (Animals : an open access journal from MDPI · 2026Article
- Comparative Muscle Quality and Multiomic Analyses in Wild-Type and Yellow-MutantFoods (Basel, Switzerland) · 2025Article
- Promoting collagen synthesis: a viable strategy to combat skin ageing.Journal of enzyme inhibition and medicinal chemistry · 2025Review
- Vitamin E Enhances Immune Function and the Intestinal Histological Structure by Regulating theBiology · 2025Article
- Vitamin E Regulates the Collagen Contents in the Body Wall of Sea Cucumber (Antioxidants (Basel, Switzerland) · 2024Article
- A signal recognition particle receptor gene from the sea cucumber, Apostichopus japonicas.Scientific reports · 2023Article
- Article
- Dietary Supplementation With Hydroxyproline Enhances Growth Performance, Collagen Synthesis and Muscle Quality ofFrontiers in physiology · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Type I collagen is proven to make an important contribution to fish muscle quality. Our previous study has shown the Smad4-dependent regulation of type I collagen expression in the muscle of crisp grass carp fed with faba bean. However, the regulatory roles of TGF-β1 or TGF-β1/Smad4 on type I collagen remain unclear in ordinary grass carp fed with normal diets or in other fish species. To clarify this point, the effect of TGF-β1 and Smad4 over-expression and RNAi knockdown on type I collagen (COL1-α1 and COL1-α2) expression were tested in vitro (zebrafish ZF4 cells) and in vivo (grass carp) along with the TGF-β1/Smad4 co-expression and co-knockdown. The mRNA levels of TGF-β1, Smad4, and type I collagen were upregulated in the groups with over-expressed TGF-β1 and Smad4 and downregulated in the groups of TGF-β1 and Smad4 RNAi in comparison to controls in vitro (P < 0.05). Similarly, in the in vivo experiment, the mRNA abundance of TGF-β1, Smad4, and type I collagen of over-expression group was higher than the controls at 36 h (P < 0.05). Co-injection of TGF-β1/Smad4 over-expression and RNAi vectors generally showed the higher efficacy. This study revealed that TGF-β1 and Smad4 genes regulated type I collagen expression in grass carp muscle and zebrafish. These findings will provide references for the collagen regulation of other freshwater fishes.
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