Evidence map›Paper›PMID 33811608›Full record

ArticleProbiotics and antimicrobial proteins2021

Isolation and Characterization of a Bacillus velezensis D-18 Strain, as a Potential Probiotic in European Seabass Aquaculture.

Luis Monzón-Atienza, Jimena Bravo, Silvia Torrecillas, Daniel Montero, Ana Franco González-de Canales, Inés García de la Banda, Jorge Galindo-Villegas, José Ramos-Vivas, Félix Acosta

Abstract read
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In one paragraph

Article in Probiotics and antimicrobial proteins, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Review
  2. Research Progress on the Effect ofCurrent issues in molecular biology · 2026
    Review
  3. Article
  4. Marine-DerivedInternational journal of molecular sciences · 2026
    Review
  5. Article
  6. Article
  7. Genomic insights into the probiotic potential ofFrontiers in microbiology · 2026
    Article
  8. Aquaculture nutrition · 2026
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
  16. Review
  17. Phage Therapy as a Focused Management Strategy in Aquaculture.International journal of molecular sciences · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Luis Monzón-AtienzaGrupo de Investigación en Acuicultura (GIA), Instituto Ecoaqua, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain.
Jimena BravoGrupo de Investigación en Acuicultura (GIA), Instituto Ecoaqua, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain.
Silvia TorrecillasGrupo de Investigación en Acuicultura (GIA), Instituto Ecoaqua, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain.
Daniel MonteroGrupo de Investigación en Acuicultura (GIA), Instituto Ecoaqua, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain.
Ana Franco González-de CanalesInstituto Español de Oceanografía, Centro Oceanográfico de Santander, Santander, Spain.
Inés García de la BandaInstituto Español de Oceanografía, Centro Oceanográfico de Santander, Santander, Spain.
Jorge Galindo-VillegasFaculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
José Ramos-VivasGrupo de Investigación en Acuicultura (GIA), Instituto Ecoaqua, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain.
Félix AcostaGrupo de Investigación en Acuicultura (GIA), Instituto Ecoaqua, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain. felix.acosta@ulpgc.es.ORCID 0000-0002-1098-7529

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Within the food-producing sectors, aquaculture is the one that has developed the greatest growth in recent decades, currently representing almost 50% of the world's edible fish. The diseases can affect the final production in intensive aquaculture; in seabass, aquaculture vibriosis is one of the most important diseases producing huge economical losses in this industry. The usual methodology to solve the problems associated with the bacterial pathology has been the use of antibiotics, with known environmental consequences. This is why probiotic bacteria are proposed as an alternative fight against pathogenic bacteria. The aim of this study was to analyse a strain of Bacillus velezensis D-18 isolated from a wastewater sample collected from a fish farm, for use as probiotics in aquaculture. The strain was evaluated in vitro through various mechanisms of selection, obtaining as results for growth inhibition by co-culture a reduction of 30%; B. velezensis D-18 was able to survive at 1.5-h exposure to 10% seabass bile, and at pH 4, its survival is 5% and reducing by 60% the adhesion capacity of V. anguillarum 507 to the mucus of seabass and in vivo by performing a challenge. Therefore, in conclusion, we consider B. velezensis D-18 isolate from wastewater samples collected from the farms as a good candidate probiotic in the prevention of the infection by Vibrio anguillarum 507 in European seabass after in vitro and biosafety assays.

Indexed as

AquacultureBacillusBassProbioticsVibrio InfectionsAnimalsVibrioWastewaterWastewaterBacillus velezensis D-18ProbioticSeabassSurvivalVibriosis

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.