ArticleBMC biology2021
Microfluidic guillotine reveals multiple timescales and mechanical modes of wound response in Stentor coeruleus.
Article in BMC biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- A role for theMolecular biology of the cell · 2026Article
- Genome-wide analysis of mRNA regionalization in a giant single cell.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mitochondrial density and cell area changes in the ciliate Paramecium bursaria under constant darkness: effects of symbiotic Chlorella variabilis and nutrient availability.Scientific reports · 2026Article
- A role for thebioRxiv : the preprint server for biology · 2025Article
- Characterization of cellular wound resistance in the giant ciliatebioRxiv : the preprint server for biology · 2025Article
- The cell biology and genome ofMolecular biology of the cell · 2025Article
- Physical Forces in Regeneration of Cells and Tissues.Cold Spring Harbor perspectives in biology · 2025Review
- Genome-wide analysis of anterior-posterior mRNA regionalization inbioRxiv : the preprint server for biology · 2024Article
- SMORES: a simple microfluidic operating room for the examination and surgery of Stentor coeruleus.Scientific reports · 2024Article
- Wound Repair of the Cell Membrane: Lessons fromCells · 2024Review
- SMORES: A Simple Microfluidic Operating Room for the Examination and Surgery ofbioRxiv : the preprint server for biology · 2024Article
- Origins of genome-editing excisases as illuminated by the somatic genome of the ciliateProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Switching of behavioral modes and their modulation by a geometrical cue in the ciliateFrontiers in cell and developmental biology · 2022Article
- Fabrication of 3D Micro-Blades for the Cutting of Biological Structures in a Microfluidic Guillotine.Micromachines · 2021Article
- Regeneration inFrontiers in cell and developmental biology · 2021Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundWound healing is one of the defining features of life and is seen not only in tissues but also within individual cells. Understanding wound response at the single-cell level is critical for determining fundamental cellular functions needed for cell repair and survival. This understanding could also enable the engineering of single-cell wound repair strategies in emerging synthetic cell research. One approach is to examine and adapt self-repair mechanisms from a living system that already demonstrates robust capacity to heal from large wounds. Towards this end, Stentor coeruleus, a single-celled free-living ciliate protozoan, is a unique model because of its robust wound healing capacity. This capacity allows one to perturb the wounding conditions and measure their effect on the repair process without immediately causing cell death, thereby providing a robust platform for probing the self-repair mechanism.
resultsHere we used a microfluidic guillotine and a fluorescence-based assay to probe the timescales of wound repair and of mechanical modes of wound response in Stentor. We found that Stentor requires ~ 100-1000 s to close bisection wounds, depending on the severity of the wound. This corresponds to a healing rate of ~ 8-80 μm
conclusionsWhile events local to the wound site have been the focus of many single-cell wound repair studies, our results suggest that large-scale mechanical behaviors may be of greater importance to single-cell wound repair than previously thought. The work here advances our understanding of the wound response in Stentor and will lay the foundation for further investigations into the underlying components and molecular mechanisms involved.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.