Evidence map›Paper›PMID 33809594›Full record

ArticleInternational journal of molecular sciences2021

Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies.

Jose Vicente Carratalá, Laia Gifre-Renom, Ramon Roca-Pinilla, Antonio Villaverde, Anna Arís, Elena Garcia-Fruitós, Julieta María Sánchez, Neus Ferrer-Miralles

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.7field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Toxicity Profiling of Bacterial Inclusion Bodies in Human Caco-2 Cells.Frontiers in bioengineering and biotechnology · 2022
    Article
  5. Coiled-Coil Based Inclusion Bodies and Their Potential Applications.Frontiers in bioengineering and biotechnology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jose Vicente CarrataláInstitute for Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain.
Laia Gifre-RenomDepartment of Ruminant Production, Institute of Agrifood Research and Technology (IRTA), Caldes de Montbui, 08140 Barcelona, Spain.ORCID 0000-0002-2115-2033
Ramon Roca-PinillaDepartment of Ruminant Production, Institute of Agrifood Research and Technology (IRTA), Caldes de Montbui, 08140 Barcelona, Spain.
Antonio VillaverdeInstitute for Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain.
Anna ArísDepartment of Ruminant Production, Institute of Agrifood Research and Technology (IRTA), Caldes de Montbui, 08140 Barcelona, Spain.ORCID 0000-0001-7830-888X
Elena Garcia-FruitósDepartment of Ruminant Production, Institute of Agrifood Research and Technology (IRTA), Caldes de Montbui, 08140 Barcelona, Spain.ORCID 0000-0001-7498-4864
Julieta María SánchezInstitute for Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain.
Neus Ferrer-MirallesInstitute for Biotechnology and Biomedicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, Spain.ORCID 0000-0003-2981-3913
Institute of Agrifood Research and Technology · ESUniversitat Autònoma de Barcelona · ES

Funding

Agència de Gestió d'Ajuts Universitaris i de Recerca 2017 SGR-229Ministerio de Economía, Industria y Competitividad, Gobierno de España RTA2015-00064-C02-01 and RTA2015-00064-C02-02
6 · The paper itself

Abstract

A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies-IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories

Indexed as

AnimalsCattleChromatography, AffinityCircular DichroismDynamic Light ScatteringEscherichia coliInclusion BodiesLactobacillusMatrix Metalloproteinase 9Protein ConformationRecombinant ProteinsSolubilitySpectrometry, FluorescenceTemperatureTryptophanMatrix Metalloproteinase 9Recombinant ProteinsTryptophanaffinity chromatographycircular dichroismdynamic light scatteringinclusion bodiesprotein conformersthe center of spectral mass

Identifiers

PMID33809594
PMCPMC8001920
OpenAlexW3135923932

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.