Evidence map›Paper›PMID 33809562›Full record

ArticleInternational journal of molecular sciences2021

Chromatography-Independent Fractionation and Newly Identified Molecular Features of the Adzuki Bean (

Biane Philadelpho, Victória Souza, Fabiani Souza, Johnnie Santos, Fabiana Batista, Mariana Silva, Jessica Capraro, Stefano De Benedetti, Giuditta C Heinzl, Eduardo Cilli and 3 more

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Soybean (International journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Biane PhiladelphoDepartment of Bromatological Analysis, School of Pharmacy, Federal University of Bahia, 40170-115 Salvador, Brazil.
Victória SouzaDepartment of Bromatological Analysis, School of Pharmacy, Federal University of Bahia, 40170-115 Salvador, Brazil.
Fabiani SouzaDepartment of Bromatological Analysis, School of Pharmacy, Federal University of Bahia, 40170-115 Salvador, Brazil.
Johnnie SantosDepartment of Bromatological Analysis, School of Pharmacy, Federal University of Bahia, 40170-115 Salvador, Brazil.
Fabiana BatistaDepartment of Bromatological Analysis, School of Pharmacy, Federal University of Bahia, 40170-115 Salvador, Brazil.
Mariana SilvaChemistry Institute, Sao Paulo State University, 14800-900 Araraquara, Brazil.
Jessica CapraroDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, Italy.ORCID 0000-0002-9114-3212
Stefano De BenedettiDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, Italy.ORCID 0000-0002-7787-9645
Giuditta C HeinzlDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, Italy.
Eduardo CilliChemistry Institute, Sao Paulo State University, 14800-900 Araraquara, Brazil.ORCID 0000-0002-4767-0904
Alessio ScarafoniDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, Italy.ORCID 0000-0003-1970-0122
Chiara MagniDepartment of Food, Environmental and Nutritional Sciences (DeFENS), Università degli Studi di Milano, 20133 Milan, Italy.ORCID 0000-0002-9641-2223
Ederlan FerreiraDepartment of Bromatological Analysis, School of Pharmacy, Federal University of Bahia, 40170-115 Salvador, Brazil.ORCID 0000-0003-3558-7019

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico Universal/CNPq, nº 426235/2016-9Fundação de Amparo à Pesquisa do Estado da Bahia nº BOL0111/2020; nº BOL2006/2019; nº BOL1867/2018; nº BOL0433/2016; nº BOL0393/2016
6 · The paper itself

Abstract

Adzuki seed β-vignin, a vicilin-like globulin, has proven to exert various health-promoting biological activities, notably in cardiovascular health. A simple scalable enrichment procedure of this protein for further nutritional and functional studies is crucial. In this study, a simplified chromatography-independent protein fractionation procedure has been optimized and described. The electrophoretic analysis showed a high degree of homogeneity of β-vignin isolate. Furthermore, the molecular features of the purified protein were investigated. The adzuki bean β-vignin was found to have a native size of 146 kDa, and the molecular weight determined was consistent with a trimeric structure. These were identified in two main polypeptide chains (masses of 56-54 kDa) that are glycosylated polypeptides with metal binding capacity, and one minor polypeptide chain with a mass 37 kDa, wherein these features are absent. The in vitro analysis showed a high degree of digestibility of the protein (92%) and potential anti-inflammatory capacity. The results lay the basis not only for further investigation of the health-promoting properties of the adzuki bean β-vignin protein, but also for a possible application as nutraceutical molecule.

Indexed as

Amino Acid SequenceCaco-2 CellsChemical FractionationChromatographyFlourGlobulinsHumansHydrogen-Ion ConcentrationInflammationPlant ProteinsSeedsSolubilityVignaGlobulinsPlant Proteinsamino acid sequencingbiological activitiesglycosylated polypeptidesin vitro digestibilitymetal binding capacityprotein fractionationprotein vicilin-type

Identifiers

PMID33809562
PMCPMC8000399

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.