Evidence map›Paper›PMID 33808507›Full record

ArticleToxins2021

Cell-Based Reporter Release Assay to Determine the Activity of Calcium-Dependent Neurotoxins and Neuroactive Pharmaceuticals.

Andrea Pathe-Neuschäfer-Rube, Frank Neuschäfer-Rube, Gerhard P Püschel

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxins, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Andrea Pathe-Neuschäfer-RubeDepartment of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, 14469 Potsdam, Germany.
Frank Neuschäfer-RubeDepartment of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, 14469 Potsdam, Germany.
Gerhard P PüschelDepartment of Nutritional Biochemistry, Institute of Nutritional Science, University of Potsdam, 14469 Potsdam, Germany.ORCID 0000-0002-0943-7060
University of Potsdam · DE

Funding

Bundesministerium für Bildung und Forschung 013L0132AEuropean Regional Development Fund 85000915
6 · The paper itself

Abstract

The suitability of a newly developed cell-based functional assay was tested for the detection of the activity of a range of neurotoxins and neuroactive pharmaceuticals which act by stimulation or inhibition of calcium-dependent neurotransmitter release. In this functional assay, a reporter enzyme is released concomitantly with the neurotransmitter from neurosecretory vesicles. The current study showed that the release of a luciferase from a differentiated human neuroblastoma-based reporter cell line (SIMA-hPOMC1-26-GLuc cells) can be stimulated by a carbachol-mediated activation of the Gq-coupled muscarinic-acetylcholine receptor and by the Ca

Indexed as

Genes, ReporterCalciumCalcium Channel BlockersCalcium ChannelsCalcium SignalingCell Line, TumorHumansLuciferasesMuscarinic AgonistsMuscarinic AntagonistsNeuroblastomaNeurotoxinsPro-OpiomelanocortinSecretory VesiclesSpider Venomsalpha-latrotoxinCalciumCalcium Channel BlockersCalcium ChannelsLuciferasesMuscarinic AgonistsMuscarinic AntagonistsNeurotoxinsPro-OpiomelanocortinSpider Venomscell-based assaymuscarinic acetylcholine receptorneurotoxinsVGCCvoltage-dependent calcium channels

Identifiers

PMID33808507
PMCPMC8066854
OpenAlexW3147981037

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.