Evidence map›Paper›PMID 33808421›Full record

ArticleInternational journal of molecular sciences2021

BODIPY Conjugate of Epibrassinolide as a Novel Biologically Active Probe for In Vivo Imaging.

Anastasiia Starodubtseva, Tetiana Kalachova, Oksana Iakovenko, Vera Stoudková, Vladimir Zhabinskii, Vladimir Khripach, Eric Ruelland, Jan Martinec, Lenka Burketová, Volodymyr Kravets

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 7 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 4 countries.

Anastasiia StarodubtsevaInstitute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague, Czech Republic.ORCID 0000-0002-8337-0837
Tetiana KalachovaInstitute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague, Czech Republic.ORCID 0000-0002-2843-5482
Oksana IakovenkoInstitute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague, Czech Republic.ORCID 0000-0003-0638-7288
Vera StoudkováInstitute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague, Czech Republic.
Vladimir ZhabinskiiInstitute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich Str., 5/2, 220141 Minsk, Belarus.ORCID 0000-0001-8591-2794
Vladimir KhripachInstitute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Kuprevich Str., 5/2, 220141 Minsk, Belarus.ORCID 0000-0002-3850-9860
Eric RuellandUMR 7025 CNRS, GEC Génie Enzymatique et Cellulaire, Centre de Recherches, Rue Personne de Roberval, CS 60319, Alliance Sorbonne Universités, Université de Technologie de Compiègne, 60203 Compiègne CEDEX, France.ORCID 0000-0002-7877-4941
Jan MartinecInstitute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague, Czech Republic.ORCID 0000-0002-5675-1706
Lenka BurketováInstitute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Prague, Czech Republic.ORCID 0000-0001-5453-5164
Volodymyr KravetsV.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, 02094 Kyiv, Ukraine.ORCID 0000-0002-6195-1665
Czech Academy of Sciences, Institute of Experimental Botany · CZNational Academy of Sciences of Belarus · BYNational Academy of Sciences of Ukraine · UAUniversité de Technologie de Compiègne · FRUniversité Paris-Est Créteil · FR

Funding

Belarusian Republican Foundation for Fundamental Research BRFFR Х20УКА-023European Regional Development Fund CZ.02.1.01/0.0/0.0/16_019/0000738International Visegrad Fund 52010701Ministerstvo Školství, Mládeže a Tělovýchovy LM2018129National Academy of Sciences of Ukraine N 13-03-20
6 · The paper itself

Abstract

Brassinosteroids (BRs) are plant hormones of steroid nature, regulating various developmental and adaptive processes. The perception, transport, and signaling of BRs are actively studied nowadays via a wide range of biochemical and genetic tools. However, most of the knowledge about BRs intracellular localization and turnover relies on the visualization of the receptors or cellular compartments using dyes or fluorescent protein fusions. We have previously synthesized a conjugate of epibrassinolide with green fluorescent dye BODIPY (eBL-BODIPY). Here we present a detailed assessment of the compound bioactivity and its suitability as probe for in vivo visualization of BRs. We show that eBL-BODIPY rapidly penetrates epidermal cells of

Indexed as

ArabidopsisBoron CompoundsBrassinosteroidsFluorescent DyesPlant Growth RegulatorsPlant RootsSignal TransductionSteroids, Heterocyclic4,4-difluoro-4-bora-3a,4a-diaza-s-indaceneBoron CompoundsbrassinolideBrassinosteroidsFluorescent DyesPlant Growth RegulatorsSteroids, Heterocyclicbrassinosteroidsfluorescent conjugateslive imagingplant bioassay

Identifiers

PMID33808421
PMCPMC8036458
OpenAlexW3142261318

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.