ArticleBiomedicines2021
Neuronal Differentiation from Induced Pluripotent Stem Cell-Derived Neurospheres by the Application of Oxidized Alginate-Gelatin-Laminin Hydrogels.
Article in Biomedicines, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Hyaluronic acid-alginate hydrazone crosslinked hydrogels support the generation and maturation of V2a interneurons.Journal of materials chemistry. B · 2026Article
- Microglia encapsulation in nanozyme-loaded hydrogels: response to oxidative stress and inflammation.Nanomedicine (London, England) · 2026Article
- Systematic investigation of the effects of neural stem cell spheroid size and density on fate specification in 3D culture.Journal of materials chemistry. B · 2026Article
- Cell Behavior and Complex Mechanical Properties of 3D Printed Cell-Laden Alginate-Gelatin Macroporous Mesostructures.Macromolecular bioscience · 2025Article
- Application of injectable hydrogels in the central and peripheral nervous system as a unique and flexible platform for neural tissue repair.Materials today. Bio · 2025Review
- A novel antibacterial hydrogel containing aminophylline as a versatile platform for neural differentiation of hWJMSCs through the CREB pathway.Scientific reports · 2025Article
- Injectable Biopolymer-Based Hydrogels: A Next-Generation Platform for Minimally Invasive Therapeutics.Gels (Basel, Switzerland) · 2025Review
- Magnesium ions regulate the Warburg effect to promote the differentiation of enteric neural crest cells into neurons.Stem cell research & therapy · 2025Article
- Hyaluronic Acid-Based 3D Bioprinted Hydrogel Structure for Directed Axonal Guidance and Modeling Innervation In Vitro.Advanced healthcare materials · 2025Article
- Nanomedicine in Neuroprotection, Neuroregeneration, and Blood-Brain Barrier Modulation: A Narrative Review.Medicina (Kaunas, Lithuania) · 2024Review
- Alginate-gelatin hydrogel promotes the neurogenic differentiation potential of bone marrow CD117Regenerative therapy · 2024Article
- Investigating the Effect of Processing and Material Parameters of Alginate Dialdehyde-Gelatin (ADA-GEL)-Based Hydrogels on Stiffness by XGB Machine Learning Model.Bioengineering (Basel, Switzerland) · 2024Article
- Effect of Electrical Stimulation on PC12 Cells Cultured in Different Hydrogels: Basis for the Development of Biomaterials in Peripheral Nerve Tissue Engineering.Pharmaceutics · 2023Article
- Article
- Novel hydrogel system eliminates subculturing and improves retention of nonsenescent mesenchymal stem cell populations.Regenerative medicine · 2023Article
- Reinforced Hyaluronic Acid-Based Matrices Promote 3D Neuronal Network Formation.Advanced healthcare materials · 2022Article
- 3D Bioprinted Neural-Like Tissue as a Platform to Study Neurotropism of Mouse-Adapted SARS-CoV-2.Advanced biology · 2022Article
- Optogenetically Engineered Neurons Differentiated from Human SH-SY5Y Cells Survived and Expressed ChR2 in 3D Hydrogel.Biomedicines · 2022Article
- Application and prospects of high-throughput screening forWorld journal of stem cells · 2022Review
- Bioorthogonally Cross-Linked Hyaluronan-Laminin Hydrogels for 3D Neuronal Cell Culture and Biofabrication.Advanced healthcare materials · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
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Abstract
Biodegradable hydrogels that promote stem cell differentiation into neurons in three dimensions (3D) are highly desired in biomedical research to study drug neurotoxicity or to yield cell-containing biomaterials for neuronal tissue repair. Here, we demonstrate that oxidized alginate-gelatin-laminin (ADA-GEL-LAM) hydrogels facilitate neuronal differentiation and growth of embedded human induced pluripotent stem cell (hiPSC) derived neurospheres. ADA-GEL and ADA-GEL-LAM hydrogels exhibiting a stiffness close to ~5 kPa at initial cell culture conditions of 37 °C were prepared. Laminin supplemented ADA-GEL promoted an increase in neuronal differentiation in comparison to pristine ADA-GEL, with enhanced neuron migration from the neurospheres to the bulk 3D hydrogel matrix. The presence of laminin in ADA-GEL led to a more than two-fold increase in the number of neurospheres with migrated neurons. Our findings suggest that laminin addition to oxidized alginate-gelatin hydrogel matrices plays a crucial role to tailor oxidized alginate-gelatin hydrogels suitable for 3D neuronal cell culture applications.
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