Evidence map›Paper›PMID 33807625›Full record

SynthesisViruses2021

Current Developments and Challenges in Plant Viral Diagnostics: A Systematic Review.

Gajanan T Mehetre, Vincent Vineeth Leo, Garima Singh, Antonina Sorokan, Igor Maksimov, Mukesh Kumar Yadav, Kalidas Upadhyaya, Abeer Hashem, Asma N Alsaleh, Turki M Dawoud and 2 more

Open access · goldAbstract readSystematic Review
In one paragraph

Synthesis in Viruses, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.

0numbers the graph read from it
0cells of the map it votes in
62citing papers in PubMed
23.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

62 citing papers in PubMed, 149 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Recent advances in characterization and diagnosis of stone fruit tree viruses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025
    Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

2 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 3 countries.

Gajanan T MehetreDepartment of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, India.
Vincent Vineeth LeoDepartment of Biotechnology, Mizoram University, Aizawl, Mizoram 796004, India.ORCID 0000-0003-1700-2259
Garima SinghDepartment of Botany, Pachhunga University College, Aizawl, Mizoram 796001, India.ORCID 0000-0002-8052-5725
Antonina SorokanInstitute of Biochemistry and Genetics, Ufa Federal Research Center of the Russian Academy of Sciences, pr. Oktyabrya 71, 450054 Ufa, Russia.
Igor MaksimovInstitute of Biochemistry and Genetics, Ufa Federal Research Center of the Russian Academy of Sciences, pr. Oktyabrya 71, 450054 Ufa, Russia.
Mukesh Kumar YadavDepartment of Biotechnology, Pachhunga University College, Aizawl, Mizoram 796001, India.
Kalidas UpadhyayaDepartment of Forestry, Mizoram University, Aizawl, Mizoram 796004, India.
Abeer HashemBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia.
Asma N AlsalehBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia.ORCID 0000-0001-9183-1552
Turki M DawoudBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia.
Khalid S AlmaaryBotany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia.
Bhim Pratap SinghDepartment of Agriculture and Environmental Sciences, National Institute of Food Technology Entrepreneurship & Management (NIFTEM), Industrial Estate, Kundli 131028, India.ORCID 0000-0002-9911-4125
King Saud University · SAMizoram University · INUfa Institute of Chemistry · RUNational Institute of Food Technology Entrepreneurship and Management · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Plant viral diseases are the foremost threat to sustainable agriculture, leading to several billion dollars in losses every year. Many viruses infecting several crops have been described in the literature; however, new infectious viruses are emerging frequently through outbreaks. For the effective treatment and prevention of viral diseases, there is great demand for new techniques that can provide accurate identification on the causative agents. With the advancements in biochemical and molecular biology techniques, several diagnostic methods with improved sensitivity and specificity for the detection of prevalent and/or unknown plant viruses are being continuously developed. Currently, serological and nucleic acid methods are the most widely used for plant viral diagnosis. Nucleic acid-based techniques that amplify target DNA/RNA have been evolved with many variants. However, there is growing interest in developing techniques that can be based in real-time and thus facilitate in-field diagnosis. Next-generation sequencing (NGS)-based innovative methods have shown great potential to detect multiple viruses simultaneously; however, such techniques are in the preliminary stages in plant viral disease diagnostics. This review discusses the recent progress in the use of NGS-based techniques for the detection, diagnosis, and identification of plant viral diseases. New portable devices and technologies that could provide real-time analyses in a relatively short period of time are prime important for in-field diagnostics. Current development and application of such tools and techniques along with their potential limitations in plant virology are likewise discussed in detail.

Indexed as

Crops, AgriculturalHigh-Throughput Nucleotide SequencingPlant DiseasesPlant VirusesPolymerase Chain ReactionVirus Diseasesdiagnosticsin-field analysisnanopore sequencingnext-generation sequencingomics technologiesplant virusesquasi-species

Identifiers

PMID33807625
PMCPMC7999175
OpenAlexW3133494080

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.