Evidence map›Paper›PMID 33806176›Full record

ArticleMicroorganisms2021

A Non-Destructive, Tuneable Method to Isolate Live Cells for High-Speed AFM Analysis.

Christopher T Evans, Sara J Baldock, John G Hardy, Oliver Payton, Loren Picco, Michael J Allen

Open access · goldAbstract read
In one paragraph

Article in Microorganisms, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 5 institutions in 2 countries.

Christopher T EvansPlymouth Marine Laboratory, Plymouth PL1 3DH, UK.
Sara J BaldockDepartment of Chemistry, Lancaster University, Lancaster LA1 4YB, UK.ORCID 0000-0003-4515-0603
John G HardyDepartment of Chemistry, Lancaster University, Lancaster LA1 4YB, UK.
Oliver PaytonInterface Analysis Centre, Wills Physics Laboratory, University of Bristol, Bristol BS8 1TL, UK.ORCID 0000-0003-0655-2167
Loren PiccoDepartment of Physics, Virginia Commonwealth University, Richmond, VA 23284, USA.
Michael J AllenPlymouth Marine Laboratory, Plymouth PL1 3DH, UK.ORCID 0000-0001-8504-7171
Lancaster University · GBPlymouth Marine Laboratory · GBUniversity of Bristol · GBUniversity of Exeter · GBVirginia Commonwealth University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Suitable immobilisation of microorganisms and single cells is key for high-resolution topographical imaging and study of mechanical properties with atomic force microscopy (AFM) under physiologically relevant conditions. Sample preparation techniques must be able to withstand the forces exerted by the Z range-limited cantilever tip, and not negatively affect the sample surface for data acquisition. Here, we describe an inherently flexible methodology, utilising the high-resolution three-dimensional based printing technique of multiphoton polymerisation to rapidly generate bespoke arrays for cellular AFM analysis. As an example, we present data collected from live

Indexed as

3D printingatomic force microscopyhigh-speedimmobilizationmicroalgaemicrobemultiphoton polymerization

Identifiers

PMID33806176
PMCPMC8066395
OpenAlexW3137498342

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.