Evidence map›Paper›PMID 33802844›Full record

ArticleAntibiotics (Basel, Switzerland)2021

Comparison of Two Transmission Electron Microscopy Methods to Visualize Drug-Induced Alterations of Gram-Negative Bacterial Morphology.

Hang Thi Nguyen, Lisa A O'Donovan, Henrietta Venter, Cecilia C Russell, Adam McCluskey, Stephen W Page, Darren J Trott, Abiodun D Ogunniyi

Abstract read
In one paragraph

Article in Antibiotics (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

  1. Effects of Tetracycline Susceptibility and Virulence in anJournal of microbiology and biotechnology · 2026
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  3. Microbiology spectrum · 2026
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  9. Engineered bacteria that self-assemble bioglass polysilicate coatings display enhanced light focusing.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  10. Article
  11. Article
  12. Article
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  14. Review
  15. Article
  16. Article
  17. Article
  18. Impact of a Novel Anticoccidial Analogue on SystemicAntibiotics (Basel, Switzerland) · 2022
    Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Hang Thi NguyenAustralia Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, Roseworthy Campus, The University of Adelaide, Roseworthy, SA 5371, Australia.
Lisa A O'DonovanARC Centre of Excellence in Plant Energy Biology, School of Agriculture Food & Wine, Waite Campus, The University of Adelaide, Urrbrae, SA 5064, Australia.ORCID 0000-0003-3154-1160
Henrietta VenterHealth and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide, SA 5000, Australia.ORCID 0000-0001-5569-7755
Cecilia C RussellChemistry School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia.
Adam McCluskeyChemistry School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia.ORCID 0000-0001-7125-863X
Stephen W PageNeoculi Pty Ltd., Burwood, VIC 3125, Australia.
Darren J TrottAustralia Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, Roseworthy Campus, The University of Adelaide, Roseworthy, SA 5371, Australia.
Abiodun D OgunniyiAustralia Centre for Antimicrobial Resistance Ecology, School of Animal and Veterinary Sciences, Roseworthy Campus, The University of Adelaide, Roseworthy, SA 5371, Australia.ORCID 0000-0001-9308-5629

Funding

Australian Research Council Linkage grant (LP110200770) to D.J.T., A.M. and S.W.P.
6 · The paper itself

Abstract

In this study, we optimized and compared different transmission electron microscopy (TEM) methods to visualize changes to Gram-negative bacterial morphology induced by treatment with a robenidine analogue (NCL195) and colistin combination. Aldehyde-fixed bacterial cells (untreated, treated with colistin or NCL195 + colistin) were prepared using conventional TEM methods and compared with ultrathin Tokuyasu cryo-sections. The results of this study indicate superiority of ultrathin cryo-sections in visualizing the membrane ultrastructure of

Indexed as

bacterial cell wallbacterial membranecolistindrug interactionGram-negative bacteriaTokuyasu cryo-ultramicrotomytransmission electron microscopy

Identifiers

PMID33802844
PMCPMC8002630

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.