Evidence map›Paper›PMID 33802273›Full record

ReviewMolecules (Basel, Switzerland)2021

Site-Specific Fluorescent Labeling of RNA Interior Positions.

Barry S Cooperman

Open access · goldAbstract readReview
In one paragraph

Review in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Barry S CoopermanDepartment of Chemistry, University of Pennsylvania, Philadelphia, PA 19104, USA.ORCID 0000-0001-6989-9788
University of Pennsylvania · US

Funding

Mechanisms of Induced Nonsense Suppression and MisreadingR01GM127374 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI BARRY S. COOPERMAN · 2018 to 2026
$2.3M
NIGMS NIH HHS R01 GM127374NIH HHS GM127374
6 · The paper itself

Abstract

The introduction of fluorophores into RNA for both in vitro and in cellulo studies of RNA function and cellular distribution is a subject of great current interest. Here I briefly review methods, some well-established and others newly developed, which have been successfully exploited to site-specifically fluorescently label interior positions of RNAs, as a guide to investigators seeking to apply this approach to their studies. Most of these methods can be applied directly to intact RNAs, including (1) the exploitation of natural posttranslational modifications, (2) the repurposing of enzymatic transferase reactions, and (3) the nucleic acid-assisted labeling of intact RNAs. In addition, several methods are described in which specifically labeled RNAs are prepared de novo.

Indexed as

AnimalsFluorescent DyesHumansOligonucleotide ProbesProtein Processing, Post-TranslationalRNAStaining and LabelingFluorescent DyesOligonucleotide ProbesRNARNA interior positionssite-specific fluorescent labeling

Identifiers

PMID33802273
PMCPMC7959133
OpenAlexW3134093407

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.