Evidence map›Paper›PMID 33801673›Full record

ArticleMetabolites2021

Optical Microscopy-Guided Laser Ablation Electrospray Ionization Ion Mobility Mass Spectrometry: Ambient Single Cell Metabolomics with Increased Confidence in Molecular Identification.

Michael J Taylor, Sara Mattson, Andrey Liyu, Sylwia A Stopka, Yehia M Ibrahim, Akos Vertes, Christopher R Anderton

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metabolites, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 33 citations in OpenAlex.

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  12. Mass Spectrometry Imaging ofJournal of the American Society for Mass Spectrometry · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Michael J TaylorEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA.ORCID 0000-0001-6926-3720
Sara MattsonDepartment of Chemistry, The George Washington University, Washington, DC 20052, USA.
Andrey LiyuEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Sylwia A StopkaDepartment of Chemistry, The George Washington University, Washington, DC 20052, USA.ORCID 0000-0003-3761-6899
Yehia M IbrahimEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA.ORCID 0000-0001-6085-193X
Akos VertesDepartment of Chemistry, The George Washington University, Washington, DC 20052, USA.ORCID 0000-0001-5186-5352
Christopher R AndertonEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA 99352, USA.ORCID 0000-0002-6170-1033
Pacific Northwest National Laboratory · USGeorge Washington University · USBrigham and Women's Hospital · US

Funding

U.S. Department of Energy DE-AC05-76RL01830
6 · The paper itself

Abstract

Single cell analysis is a field of increasing interest as new tools are continually being developed to understand intercellular differences within large cell populations. Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) is an emerging technique for single cell metabolomics. Over the years, it has been validated that this ionization technique is advantageous for probing the molecular content of individual cells in situ. Here, we report the integration of a microscope into the optical train of the LAESI source to allow for visually informed ambient in situ single cell analysis. Additionally, we have coupled this 'LAESI microscope' to a drift-tube ion mobility mass spectrometer to enable separation of isobaric species and allow for the determination of ion collision cross sections in conjunction with accurate mass measurements. This combined information helps provide higher confidence for structural assignment of molecules ablated from single cells. Here, we show that this system enables the analysis of the metabolite content of

Indexed as

ambient analysiscollisional cross sectiondrift tube ion mobility separationin situ metabolomicslaser ablation electrospray ionizationmass spectrometry

Identifiers

PMID33801673
PMCPMC8065410
OpenAlexW3149405437

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.