Evidence map›Paper›PMID 33801034›Full record

ArticlePlants (Basel, Switzerland)2021

Non-Uniform Distribution of Cryoprotecting Agents in Rice Culture Cells Measured by CARS Microscopy.

Fionna M D Samuels, Dominik G Stich, Remi Bonnart, Gayle M Volk, Nancy E Levinger

Open access · goldAbstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Tracking Permeation of Dimethyl Sulfoxide (DMSO) inPlants (Basel, Switzerland) · 2023
    Article
  4. Article
  5. Cryopreservation of 13 CommercialPlants (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Fionna M D SamuelsDepartment of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
Dominik G StichAdvanced Light Microscopy Core, NeuroTechnology Center, University of Colorado School of Medicine, Anschutz Medical Campus, Aurora, CO 80045, USA.
Remi BonnartUSDA-ARS National Laboratory for Genetic Resources Preservation, 1111 S. Mason St., Fort Collins, CO 80521, USA.
Gayle M VolkUSDA-ARS National Laboratory for Genetic Resources Preservation, 1111 S. Mason St., Fort Collins, CO 80521, USA.
Nancy E LevingerDepartment of Chemistry, Colorado State University, Fort Collins, CO 80523, USA.
Colorado State University · USUniversity of Colorado Anschutz Medical Campus · US

Funding

University of Colorado Anschutz Medical Campus DRCP30DK116073 · NIDDK · UNIVERSITY OF COLORADO DENVER · PI PETER J DEMPSEY · 2020 to 2026
$10.8M
ZEBRAFISH TRANSGENIC CORE - ROCKY MOUNTAIN NEUROLOGICAL DISORDERS CORE CENTERP30NS048154 · NINDS · UNIVERSITY OF COLORADO DENVER · PI RIBERA, ANGELES BADELL · 2004 to 2020
$10.1M
National Institute of Food and Agriculture 12835363NIDDK NIH HHS P30 DK116073NINDS NIH HHS P30 NS048154NSF Graduate Research Fellowship 2019273956
6 · The paper itself

Abstract

Cryoprotectants allow cells to be frozen in liquid nitrogen and cryopreserved for years by minimizing the damage that occurs in cooling and warming processes. Unfortunately, how the specific cryoprotectants keep the cells viable through the cryopreservation process is not entirely evident. This contributes to the arduous process of optimizing cryoprotectant formulations for each new cell line or species that is conserved. Coherent anti-Stokes Raman scattering microscopy facilitates the visualization of deuterated cryoprotectants within living cells. Using this technique, we directly imaged the location of fully deuterated dimethyl sulfoxide (d

Indexed as

cryopreservationcryoprotectant distributionraman microscopy

Identifiers

PMID33801034
PMCPMC8003852
OpenAlexW3136748585

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.