ArticleMolecular and cellular biology1988
Poly(A) shortening and degradation of the 3' A+U-rich sequences of human c-myc mRNA in a cell-free system.
Article in Molecular and cellular biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers.
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Who cites it
116 citing papers in PubMed, 322 citations in OpenAlex.
- Cytoplasmic poly-adenosine binding proteins modulate susceptibility of mRNAs to Pumilio-mediated decay.Nucleic acids research · 2026Article
- Cytoplasmic poly-adenosine binding proteins modulate susceptibility of mRNAs to RNA-binding protein-directed decay.bioRxiv : the preprint server for biology · 2025Article
- Mathematical Modeling of mRNA Poly(A) Tail Shortening Process.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Roles of mRNA poly(A) tails in regulation of eukaryotic gene expression.Nature reviews. Molecular cell biology · 2022Review
- Merkel cell polyomavirus T-antigens regulateiScience · 2021Article
- High-throughput 5'P sequencing enables the study of degradation-associated ribosome stalls.Cell reports methods · 2021Article
- Pumilio response and AU-rich elements drive rapid decay of Pnrc2-regulated cyclic gene transcripts.Developmental biology · 2020Article
- Therapeutic Inhibition of Myc in Cancer. Structural Bases and Computer-Aided Drug Discovery Approaches.International journal of molecular sciences · 2018Review
- A Plant Biologist's Toolbox to Study Translation.Frontiers in plant science · 2018Review
- RNA Degradation in Neurodegenerative Disease.Advances in neurobiology · 2018Review
- Transcribed ultraconserved region 339 promotes carcinogenesis by modulating tumor suppressor microRNAs.Nature communications · 2017Article
- Human cellular CYBA UTR sequences increase mRNA translation without affecting the half-life of recombinant RNA transcripts.Scientific reports · 2016Article
- Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs.RNA (New York, N.Y.) · 2009Article
- Chapter 3. Assays of adenylate uridylate-rich element-mediated mRNA decay in cells.Methods in enzymology · 2008Article
- Assembly of AUF1 with eIF4G-poly(A) binding protein complex suggests a translation function in AU-rich mRNA decay.RNA (New York, N.Y.) · 2006Article
- AU-rich elements and associated factors: are there unifying principles?Nucleic acids research · 2005Review
- The herpes simplex virus 1 UL41 gene-dependent destabilization of cellular RNAs is selective and may be sequence-specific.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The KH-domain protein alpha CP has a direct role in mRNA stabilization independent of its cognate binding site.Molecular and cellular biology · 2003Article
- MRNA stability and the control of gene expression: implications for human disease.Neurochemical research · 2002Review
- Cytoplasmic-nuclear shuttling of the urokinase mRNA binding protein regulates message stability.Molecular and cellular biochemistry · 2002Article
56 more citing papers are in PubMed but not listed here.
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2 authors at 1 institution in 1 country.
Funding
Abstract
The early steps in the degradation of human c-myc mRNA were investigated, using a previously described cell-free mRNA decay system. The first detectable step was poly(A) shortening, which generated a pool of oligoadenylated mRNA molecules. In contrast, the poly(A) of a stable mRNA, gamma globin, was not excised, even after prolonged incubation. The second step, degradation of oligoadenylated c-myc mRNA, generated decay products whose 3' termini were located within the A+U-rich portion of the 3' untranslated region. These products disappeared soon after they were formed, consistent with rapid degradation of the 3' region. In contrast, the 5' region, corresponding approximately to c-myc exon 1, was stable in vitro. The data indicate a sequential degradation pathway in which 3' region cleavages occur only after most or all of the poly(A) is removed. To account for rapid deadenylation, we suggest that the c-myc poly(A)-poly(A)-binding protein complex is readily dissociated, generating a protein-depleted poly(A) tract that is no longer resistant to nucleases.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.