Evidence map›Paper›PMID 33800884›Full record

ArticleInternational journal of molecular sciences2021

Sofosbuvir Selects for Drug-Resistant Amino Acid Variants in the Zika Virus RNA-Dependent RNA-Polymerase Complex In Vitro.

Adele Boccuto, Filippo Dragoni, Francesca Picarazzi, Alessia Lai, Carla Della Ventura, Carla Veo, Federica Giammarino, Francesco Saladini, Gianguglielmo Zehender, Maurizio Zazzi and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Adele BoccutoDepartment of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.ORCID 0000-0003-4054-2181
Filippo DragoniDepartment of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.ORCID 0000-0002-8148-9914
Francesca PicarazziDepartment of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy.ORCID 0000-0003-3585-4228
Alessia LaiDepartment of Biomedical and Clinical Sciences L. Sacco, University of Milan, 20157 Milan, Italy.
Carla Della VenturaDepartment of Biomedical and Clinical Sciences L. Sacco, University of Milan, 20157 Milan, Italy.
Carla VeoClinical Pathology Analysis Laboratory ASL Taranto, 74023 Taranto, Italy.
Federica GiammarinoDepartment of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.
Francesco SaladiniDepartment of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.
Gianguglielmo ZehenderDepartment of Biomedical and Clinical Sciences L. Sacco, University of Milan, 20157 Milan, Italy.ORCID 0000-0002-1886-2915
Maurizio ZazziDepartment of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.ORCID 0000-0002-0344-6281
Mattia MoriDepartment of Biotechnology, Chemistry and Pharmacy, University of Siena, 53100 Siena, Italy.ORCID 0000-0003-2398-1254
Ilaria VicentiDepartment of Medical Biotechnologies, University of Siena, 53100 Siena, Italy.ORCID 0000-0002-4306-2960
University of Siena · ITUniversity of Milan · IT

Funding

Ministero dell'Istruzione, dell'Università e della Ricerca cod. 2017BMK8JR
6 · The paper itself

Abstract

The nucleotide analog sofosbuvir, licensed for the treatment of hepatitis C, recently revealed activity against the Zika virus (ZIKV) in vitro and in animal models. However, the ZIKV genetic barrier to sofosbuvir has not yet been characterized. In this study, in vitro selection experiments were performed in infected human hepatoma cell lines. Increasing drug pressure significantly delayed viral breakthrough (

Indexed as

Amino Acid SubstitutionPoint MutationAnimalsAntiviral AgentsBinding SitesCarcinoma, HepatocellularCell Line, TumorChlorocebus aethiopsHumansInhibitory Concentration 50Liver NeoplasmsModels, MolecularMolecular Dynamics SimulationProtein BindingProtein ConformationRNA-Dependent RNA PolymeraseAntiviral AgentsPOLY protein, Zika virusRNA-Dependent RNA PolymeraseRNA, ViralSofosbuvirViral Nonstructural Proteinsgenetic barrierin vitro selectionmolecular dynamicssofosbuvirZika virus

Identifiers

PMID33800884
PMCPMC7962015
OpenAlexW3134351272

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.