ReviewInternational journal of molecular sciences2021
Label-Free Multiphoton Microscopy: Much More Than Fancy Images.
Review in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- Detecting drug-induced nephrotoxicity using simultaneous label-free autofluorescence multiharmonic microscopy.Toxicological sciences : an official journal of the Society of Toxicology · 2026Article
- Deep Learning Integration in Optical Microscopy: Advancements and Applications.Microscopy research and technique · 2026Review
- Label-free multiphoton microscopy and machine learning for recognition of hepatocellular carcinoma.Scientific reports · 2026Article
- Challenges and Prospects of Using Novel Nonlinear Effects in Multimode Optical Fibers for Multiphoton Endomicroscopy.Diagnostics (Basel, Switzerland) · 2026Review
- New frontiers in porcine atrioventricular node decellularization: preserving extracellular matrix architecture for biological scaffolds.Frontiers in bioengineering and biotechnology · 2026Article
- Assessment of Collagen and Fibroblast Properties via Label-Free Higher Harmonic Generation Microscopy in Three-Dimensional Models of Osteogenesis Imperfecta and Ehlers-Danlos Syndrome.International journal of molecular sciences · 2025Article
- Comparative Analysis of Reflectance Confocal Microscopy and Multiphoton Microscopy in Basal Cell Carcinoma Diagnosis.Journal of biophotonics · 2025Article
- Investigating machine learning algorithms to classify label-free images of pancreatic neuroendocrine neoplasms.Biophotonics discovery · 2025Article
- Three-dimensional quantitative evaluation of hypertension-induced aortic fibre remodelling based on multiphoton microscopy: a cross-age perspective.Royal Society open science · 2025Article
- A novel histological occlusion classification for coiled aneurysms based on multiphoton microscopy.Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences · 2025Article
- Quantitative Stain-Free Conjunctival Collagen Imaging in Cicatrizing Conjunctivitis Using Second Harmonic Generation-Two Photon Excitation Technology.Investigative ophthalmology & visual science · 2025Article
- Visualizing cellular interactions: intravital imaging in tumor microenvironment.Frontiers in immunology · 2025Review
- Seeing the unseen: The role of bioimaging techniques for the diagnostic interventions in intervertebral disc degeneration.Bone reports · 2024Review
- Low-Frequency Coherent Raman Imaging Robust to Optical Scattering.Chemical & biomedical imaging · 2024Article
- Hybrid Materials for Vascular Applications: A Preliminary In Vitro Assessment.Bioengineering (Basel, Switzerland) · 2024Article
- Numerical study of transient absorption saturation in single-layer graphene for optical nanoscopy applications.Scientific reports · 2024Article
- The suprapatellar fat pad: A histotopographic comparative study.Journal of anatomy · 2024Article
- Two-photon excitation fluorescence microspectroscopy protocols for examining fluorophores in fossil plants.Communications biology · 2024Article
- Label-free multimodal imaging with simultaneous two-photon and three-photon microscopy and kernel-based nonlinear scaling denoising.Biomedical optics express · 2024Article
- Imagining the future of optical microscopy: everything, everywhere, all at once.Communications biology · 2023Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Multiphoton microscopy has recently passed the milestone of its first 30 years of activity in biomedical research. The growing interest around this approach has led to a variety of applications from basic research to clinical practice. Moreover, this technique offers the advantage of label-free multiphoton imaging to analyze samples without staining processes and the need for a dedicated system. Here, we review the state of the art of label-free techniques; then, we focus on two-photon autofluorescence as well as second and third harmonic generation, describing physical and technical characteristics. We summarize some successful applications to a plethora of biomedical research fields and samples, underlying the versatility of this technique. A paragraph is dedicated to an overview of sample preparation, which is a crucial step in every microscopy experiment. Afterwards, we provide a detailed review analysis of the main quantitative methods to extract important information and parameters from acquired images using second harmonic generation. Lastly, we discuss advantages, limitations, and future perspectives in label-free multiphoton microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.