Evidence map›Paper›PMID 33799994›Full record

ArticleInternational journal of molecular sciences2021

A2A Receptor Dysregulation in Dystonia DYT1 Knock-Out Mice.

Vincenza D'Angelo, Mauro Giorgi, Emanuela Paldino, Silvia Cardarelli, Francesca R Fusco, Ilaria Saverioni, Roberto Sorge, Giuseppina Martella, Stefano Biagioni, Nicola B Mercuri and 2 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review of Preclinical and Clinical Studies Supporting the Role of Polydeoxyribonucleotide in the Treatment of Tendon Disorders.Medical science monitor : international medical journal of experimental and clinical research · 2025
    Review
  3. Article
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  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Vincenza D'AngeloDepartment of Systems Medicine, Tor Vergata University of Rome, 00133 Rome, Italy.ORCID 0000-0002-7720-8913
Mauro GiorgiDepartment of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy.
Emanuela PaldinoIRCCS Santa Lucia Foundation, 00179 Rome, Italy.
Silvia CardarelliDepartment of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy.
Francesca R FuscoIRCCS Santa Lucia Foundation, 00179 Rome, Italy.
Ilaria SaverioniDepartment of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy.
Roberto SorgeDepartment of Systems Medicine, Tor Vergata University of Rome, 00133 Rome, Italy.
Giuseppina MartellaDepartment of Systems Medicine, Tor Vergata University of Rome, 00133 Rome, Italy.ORCID 0000-0002-8927-7107
Stefano BiagioniDepartment of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0003-1544-3907
Nicola B MercuriDepartment of Systems Medicine, Tor Vergata University of Rome, 00133 Rome, Italy.
Antonio PisaniIRCCS Mondino Foundation, 27100 Pavia, Italy.ORCID 0000-0002-8432-594X
Giuseppe SancesarioDepartment of Systems Medicine, Tor Vergata University of Rome, 00133 Rome, Italy.
University of Rome Tor Vergata · ITSapienza University of Rome · ITFondazione Santa Lucia · ITUniversity of Pavia · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We aimed to investigate A2A receptors in the basal ganglia of a DYT1 mouse model of dystonia. A2A was studied in control Tor1a+/+ and Tor1a+/- knock-out mice. A2A expression was assessed by anti-A2A antibody immunofluorescence and Western blotting. The co-localization of A2A was studied in striatal cholinergic interneurons identified by anti-choline-acetyltransferase (ChAT) antibody. A2A mRNA and cyclic adenosine monophosphate (cAMP) contents were also assessed. In Tor1a+/+, Western blotting detected an A2A 45 kDa band, which was stronger in the striatum and the globus pallidus than in the entopeduncular nucleus. Moreover, in Tor1a+/+, immunofluorescence showed A2A roundish aggregates, 0.3-0.4 μm in diameter, denser in the neuropil of the striatum and the globus pallidus than in the entopeduncular nucleus. In Tor1a+/-, A2A Western blotting expression and immunofluorescence aggregates appeared either increased in the striatum and the globus pallidus, or reduced in the entopeduncular nucleus. Moreover, in Tor1a+/-, A2A aggregates appeared increased in number on ChAT positive interneurons compared to Tor1a+/+. Finally, in Tor1a+/-, an increased content of cAMP signal was detected in the striatum, while significant levels of A2A mRNA were neo-expressed in the globus pallidus. In Tor1a+/-, opposite changes of A2A receptors' expression in the striatal-pallidal complex and the entopeduncular nucleus suggest that the pathophysiology of dystonia is critically dependent on a composite functional imbalance of the indirect over the direct pathway in basal ganglia.

Indexed as

AnimalsBasal GangliaCholinergic NeuronsCorpus StriatumCyclic AMPDisease Models, AnimalDystonia Musculorum DeformansGene Expression RegulationMaleMiceMice, Inbred C57BLMice, KnockoutMicroscopy, FluorescenceMolecular ChaperonesReceptor, Adenosine A2ARNA, MessengerAdora2a protein, mouseCyclic AMPDyt1 protein, mouseMolecular ChaperonesReceptor, Adenosine A2ARNA, MessengerA2AA2A mRNAbasal gangliacAMPD2dystoniaDYT1

Identifiers

PMID33799994
PMCPMC7962104
OpenAlexW3133831734

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.