ArticleMolecules (Basel, Switzerland)2021
Zymography for Picogram Detection of Lipase and Esterase Activities.
Article in Molecules (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- Heterologous expression of lipase gene in Cereibacter sphaeroides 2.4.1 driven by an anaerobic Photosynthesis-Inducible promoter.Journal, genetic engineering & biotechnology · 2026Article
- Cloning and Characterization of GDSL Esterases fromBiology · 2026Article
- Glycosidase Isoforms in Honey and the Honey Bee (Insects · 2025Article
- Abridgement of Microbial Esterases and Their Eminent Industrial Endeavors.Molecular biotechnology · 2025Review
- Applying Zymography Methods for the Detection of Enzymes from Fungal Endophytes.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Polyester degradation by soil bacteria: identification of conserved BHETase enzymes in Streptomyces.Communications biology · 2024Article
- Screening, identification, and characterization of lipase-producing halotolerant Bacillus altitudinis Ant19 from Antarctic soil.Archives of microbiology · 2023Article
- A Novel Lipase fromInternational journal of molecular sciences · 2021Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipases and esterases are important catalysts with wide varieties of industrial applications. Although many methods have been established for detecting their activities, a simple and sensitive approach for picogram detection of lipolytic enzyme quantity is still highly desirable. Here we report a lipase detection assay which is 1000-fold more sensitive than previously reported methods. Our assay enables the detection of as low as 5 pg and 180 pg of lipolytic activity by direct spotting and zymography, respectively. Furthermore, we demonstrated that the detection sensitivity was adjustable by varying the buffering capacity, which allows for screening of both high and low abundance lipolytic enzymes. Coupled with liquid chromatography-mass spectrometry, our method provides a useful tool for sensitive detection and identification of lipolytic enzymes.
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