Evidence map›Paper›PMID 33798277›Full record

ArticleCurrent protocols2021

Culturing and Multiplexed Time-Lapse Imaging of Fungal Isolates from Marine and Coastal Environments.

Lorna M Y Mitchison-Field, Amy S Gladfelter

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Current protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Journal of fungi (Basel, Switzerland) · 2024
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Lorna M Y Mitchison-FieldMarine Biological Laboratory, Woods Hole, Massachusetts.
Amy S GladfelterMarine Biological Laboratory, Woods Hole, Massachusetts.

Funding

NSF MCB 2016022NSF MCB GBMF9343
6 · The paper itself

Abstract

Fungi play a crucial role in biogeochemical cycling and shaping biological communities at macro- and microcosmic scales. However, fungi have been largely overlooked in studies of marine ecology and microbiology. Here we present protocols for preparing culture media, collecting and culturing fungi from several types of marine environments and animal hosts, and preparing microscopy slides for long-term time lapse imaging of fungal isolates. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Solid media preparation Alternate Protocol 1: Liquid media preparation Basic Protocol 2: Sample collection and plating from ocean water Alternate Protocol 2: Sample collection and plating from coastal sediment Alternate Protocol 3: Sample collection and plating from animal holobionts Support Protocol 1: Making frozen stocks Support Protocol 2: Streaking cultures from frozen stocks Basic Protocol 3: Slide preparation and assembly for imaging © 2021 by John Wiley & Sons, Inc.

Indexed as

BiodiversityFungiAnimalsCulture MediaSeawaterTime-Lapse ImagingCulture Mediaenvironmental microbiologymarine fungimicroscopymycology

Identifiers

PMID33798277

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.