Evidence map›Paper›PMID 33797851›Full record

ArticleAngewandte Chemie (International ed. in English)2021

High-Throughput Counting and Superresolution Mapping of Tetraspanins on Exosomes Using a Single-Molecule Sensitive Flow Technique and Transistor-like Semiconducting Polymer Dots.

Yifei Jiang, Luca A Andronico, Seung-Ryoung Jung, Haobin Chen, Bryant Fujimoto, Lucia Vojtech, Daniel T Chiu

Open access · greenAbstract read
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 51 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yifei JiangDepartment of Chemistry and Bioengineering, University of Washington, Seattle, Washington, 98195, USA.ORCID 0000-0003-1818-4541
Luca A AndronicoDepartment of Chemistry and Bioengineering, University of Washington, Seattle, Washington, 98195, USA.
Seung-Ryoung JungDepartment of Chemistry and Bioengineering, University of Washington, Seattle, Washington, 98195, USA.
Haobin ChenDepartment of Chemistry and Bioengineering, University of Washington, Seattle, Washington, 98195, USA.
Bryant FujimotoDepartment of Chemistry and Bioengineering, University of Washington, Seattle, Washington, 98195, USA.
Lucia VojtechDepartment of Obstetrics and Gynecology, University of Washington, Seattle, Washington, 98195, USA.
Daniel T ChiuDepartment of Chemistry and Bioengineering, University of Washington, Seattle, Washington, 98195, USA.ORCID 0000-0003-2964-9578
University of Washington · US

Funding

MicroRNAs in CNS-derived extracellular microvesicles as peripheral blood biomarkers for Alzheimer diseaseR01AG061383 · NIA · UNIVERSITY OF WASHINGTON · PI SHI, MIN · 2019 to 2023
$3.4M
Extracellular vesicle transport of brain-derived proteins to the blood in Alzheimer diseaseRF1AG068406 · NIA · UNIVERSITY OF WASHINGTON · PI SHI, MIN · 2020 to 2020
$3.0M
Characterization and quantification of CNS cell specific extracellular microvesicles in bloodR33MH118160 · NIMH · UNIVERSITY OF WASHINGTON · PI SHI, MIN · 2020 to 2022
$2.0M
Single Extracellular Vesicle Sorting and AnalysisUH3TR002874 · NCATS · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T · 2021 to 2022
$2.0M
High-precision mapping of the spatial organization of synaptic-vesicle membrane proteinsR01MH113333 · NIMH · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T · 2017 to 2021
$1.9M
Single Extracellular Vesicle Sorting and AnalysisUG3TR002874 · NCATS · UNIVERSITY OF WASHINGTON · PI CHIU, DANIEL T · 2019 to 2020
$813k
Extracellular vesicle transport of brain-derived proteins to the blood in Alzheimer diseaseR01AG068406 · NIA · UNIVERSITY OF WASHINGTON · PI SHI, MIN · 2024 to 2024
$704k
NCATS NIH HHS UG3 TR002874NCATS NIH HHS UH3 TR002874NIA NIH HHS R01 AG061383NIA NIH HHS R01 AG068406NIA NIH HHS RF1 AG068406NIMH NIH HHS R01 MH113333NIMH NIH HHS R33 MH118160
6 · The paper itself

Abstract

A method for high-throughput counting and superresolution mapping of surface proteins on exosomes is described. The method combines a single-molecule sensitive flow technique and an adaptive superresolution imaging method. Exosomes stained with membrane dye and dye-conjugated antibodies were analyzed using a microfluidic platform at a flow rate of 100 exosome s

Indexed as

AntibodiesExosomesFluorescent DyesHigh-Throughput Screening AssaysHumansMicrofluidicsPolymersQuantum DotsSemiconductorsTetraspaninsAntibodiesFluorescent DyesPolymersTetraspaninsexosomesfluorescent probesmembrane proteinsmicrofluidicssuperresolution imaging

Identifiers

PMID33797851
PMCPMC8215978
OpenAlexW3150425827

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.