ArticleBiotechnology letters2021
Multiple gene integration to promote erythritol production on glycerol in Yarrowia lipolytica.
Article in Biotechnology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Integrated Molecular and Hematobiochemical Biomarkers for the Detection of Bovine Babesiosis in Holstein Calves.Veterinary sciences · 2026Article
- Nucleotide sequence variations, gene expression profiles, and host biomarkers associated with ovine mange caused byFrontiers in veterinary science · 2026Article
- Variants in Nucleotide Sequences; Gene Expression; and Hematological, Immune, and Antioxidant Biomarkers Linked to Pneumonia Risk in Holstein Calves.Veterinary sciences · 2025Article
- Comparative Transcriptome Analysis Reveals Key Genes Related to Erythritol Production inInternational journal of molecular sciences · 2025Article
- Synergistic effect of transporter and pathway engineering on the key performance indicators of erythritol synthesis by the yeastApplied and environmental microbiology · 2025Article
- Biosynthesis and metabolic engineering of natural sweeteners.AMB Express · 2025Review
- Glycerol bioconversion to biofuel and value-added products by yeasts.FEMS yeast research · 2025Review
- Nucleotide sequence variants, gene expression and serum profile of immune and antioxidant markers associated with brucellosis resistance/susceptibility in Shami goat.Irish veterinary journal · 2025Article
- Advances and perspectives in genetic expression and operation for the oleaginous yeastSynthetic and systems biotechnology · 2024Review
- Research progress on biosynthesis of erythritol and multi-dimensional optimization of production strategies.World journal of microbiology & biotechnology · 2024Review
- Application of NewInternational journal of molecular sciences · 2024Article
- Construction of a xylose metabolic pathway in Trichosporonoides oedocephalis ATCC 16958 for the production of erythritol and xylitol.Biotechnology letters · 2023Article
- Multiplex modification of Yarrowia lipolytica for enhanced erythritol biosynthesis from glycerol through modularized metabolic engineering.Bioprocess and biosystems engineering · 2023Article
- Investigating Polymorphisms and Expression Profile of Immune, Antioxidant, and Erythritol-Related Genes for Limiting Postparturient Endometritis in Holstein Cattle.Veterinary sciences · 2023Article
- Microencapsulation of Yarrowia lipolytica: cell viability and application in vitro ruminant diets.World journal of microbiology & biotechnology · 2023Article
- Cell Immobilization for Erythritol Production.Journal of fungi (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
objectiveErythritol (1,2,3,4-butanetetrol) is a 4-carbon sugar alcohol that occurs in nature as a metabolite or storage compound. In this study, a multiple gene integration strategy was employed to enhance erythritol production in Y. lipolytica.
resultsThe effects on the production of erythritol in Y. lipolytica of seven key genes involved in the erythritol synthesis pathway were evaluated individually, among which transketolase (TKL1) and transaldolase (TAL1) showed important roles in enhancing erythritol production. The combined overexpression of four genes (GUT1, TPI1, TKL1, TAL1) and disruption of the EYD1 gene (encoding erythritol dehydrogenase), resulted in produce approximately 40 g/L erythritol production from glycerol. Further enhanced erythritol synthesis was obtained by overexpressing the RKI1 gene (encoding ribose 5-phosphate isomerase) and the AMPD gene (encoding AMP deaminase), indicating for the first time that these two genes are also related to the enhancement of erythritol production in Y. lipolytica.
conclusionsA combined gene overexpression strategy was developed to efficiently improve the production of erythritol in Y. lipolytica, suggesting a great capacity and promising potential of this non-conventional yeast in converting glycerol into erythritol.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.