Evidence map›Paper›PMID 33797618›Full record

ArticleMikrochimica acta2021

Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles.

Pavel Pořízka, Karolína Vytisková, Radka Obořilová, Matěj Pastucha, Ivo Gábriš, Julian C Brandmeier, Pavlína Modlitbová, Hans H Gorris, Karel Novotný, Petr Skládal and 2 more

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 23 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Pavel PořízkaCentral European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Karolína VytiskováDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Radka ObořilováDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Matěj PastuchaDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Ivo GábrišDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Julian C BrandmeierInstitute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93040, Regensburg, Germany.
Pavlína ModlitbováCentral European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Hans H GorrisInstitute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, Universitätsstraße 31, 93040, Regensburg, Germany.
Karel NovotnýDepartment of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic. codl@sci.muni.cz.ORCID 0000-0002-3319-2411
Petr SkládalDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Jozef KaiserCentral European Institute of Technology, Brno University of Technology, Purkyňova 656/123, 612 00, Brno, Czech Republic.
Zdeněk FarkaDepartment of Biochemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00, Brno, Czech Republic.
Central European Institute of Technology · CZMasaryk University · CZUniversity of Regensburg · DE

Funding

Grantová Agentura České Republiky 20-19526YMinisterstvo Školství, Mládeže a Tělovýchovy LM2018110Ministerstvo Školství, Mládeže a Tělovýchovy LM2018127Ministerstvo Školství, Mládeže a Tělovýchovy LQ1601Ministerstvo Školství, Mládeže a Tělovýchovy LTAB19011Technologická Agentura České Republiky GJ20-30004Y
6 · The paper itself

Abstract

Immunohistochemistry (IHC) and immunocytochemistry (ICC) are widely used to identify cancerous cells within tissues and cell cultures. Even though the optical microscopy evaluation is considered the gold standard, the limited range of useful labels and narrow multiplexing capabilities create an imminent need for alternative readout techniques. Laser-induced breakdown spectroscopy (LIBS) enables large-scale multi-elemental analysis of the surface of biological samples, e.g., thin section or cell pellet. It is, therefore, a potential alternative for IHC and ICC readout of various labels or tags (Tag-LIBS approach). Here, we introduce Tag-LIBS as a method for the specific determination of HER2 biomarker. The cell pellets were labeled with streptavidin-conjugated upconversion nanoparticles (UCNP) through a primary anti-HER2 antibody and a biotinylated secondary antibody. The LIBS scanning enabled detecting the characteristic elemental signature of yttrium as a principal constituent of UCNP, thus indirectly providing a reliable way to differentiate between HER2-positive BT-474 cells and HER2-negative MDA-MB-231 cells. The comparison of results with upconversion optical microscopy and luminescence intensity scanning confirmed that LIBS is a promising alternative for the IHC and ICC readout.

Indexed as

Antibodies, ImmobilizedBiomarkers, TumorCell Line, TumorErb-b2 Receptor Tyrosine KinasesFeasibility StudiesFluoridesHumansImmunohistochemistryLightNanoparticlesSpectrum AnalysisThuliumYttriumAntibodies, ImmobilizedBiomarkers, TumorERBB2 protein, humanErb-b2 Receptor Tyrosine KinasesFluoridessodium yttriumtetrafluorideThuliumYttriumImmunocytochemistryImmunohistochemistryLaser-induced breakdown spectroscopyPhoton-upconversion nanoparticlesTag-LIBS

Identifiers

PMID33797618
OpenAlexW3148120773

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.