Evidence map›Paper›PMID 33797236›Full record

ArticleJournal of the American Chemical Society2021

Stealth Fluorescence Labeling for Live Microscopy Imaging of mRNA Delivery.

Tom Baladi, Jesper R Nilsson, Audrey Gallud, Emanuele Celauro, Cécile Gasse, Fabienne Levi-Acobas, Ivo Sarac, Marcel R Hollenstein, Anders Dahlén, Elin K Esbjörner and 1 more

Open access · hybridAbstract read
In one paragraph

Article in Journal of the American Chemical Society, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 44 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Imaging methods to monitor and quantify cell differentiation.Frontiers in cell and developmental biology · 2025
    Review
  7. Article
  8. Article
  9. Article
  10. Review
  11. Review
  12. Isomorphic Fluorescent Nucleosides.Accounts of chemical research · 2024
    Article
  13. Article
  14. Review
  15. Generation and Characterization of In Vitro Transcribed mRNA.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Tom BaladiDepartment of Chemistry and Chemical Engineering, Chemistry and Biochemistry, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.ORCID 0000-0003-3421-9417
Jesper R NilssonDepartment of Chemistry and Chemical Engineering, Chemistry and Biochemistry, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.
Audrey GalludDepartment of Biology and Biological Engineering, Chemical Biology, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.
Emanuele CelauroDepartment of Biology and Biological Engineering, Chemical Biology, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.
Cécile GasseGénomique Métabolique, Genoscope, Institut François Jacob, CEA, CNRS, Univ Evry, Université Paris-Saclay, 91057 Evry, France.
Fabienne Levi-AcobasDepartment of Structural Biology and Chemistry, Laboratory for Bioorganic Chemistry of Nucleic Acids, CNRS UMR3523, Institut Pasteur, 28, Rue du Docteur Roux, 75724 Paris CEDEX 15, France.
Ivo SaracDepartment of Structural Biology and Chemistry, Laboratory for Bioorganic Chemistry of Nucleic Acids, CNRS UMR3523, Institut Pasteur, 28, Rue du Docteur Roux, 75724 Paris CEDEX 15, France.
Marcel R HollensteinDepartment of Structural Biology and Chemistry, Laboratory for Bioorganic Chemistry of Nucleic Acids, CNRS UMR3523, Institut Pasteur, 28, Rue du Docteur Roux, 75724 Paris CEDEX 15, France.ORCID 0000-0003-0263-9206
Anders DahlénMedicinal Chemistry, Research and Early Development, Cardiovascular, Renal and Metabolism, BioPharmaceuticals R&D, AstraZeneca, Gothenburg, Sweden.
Elin K EsbjörnerDepartment of Biology and Biological Engineering, Chemical Biology, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.ORCID 0000-0002-1253-6342
L Marcus WilhelmssonDepartment of Chemistry and Chemical Engineering, Chemistry and Biochemistry, Chalmers University of Technology, SE-41296 Gothenburg, Sweden.ORCID 0000-0002-2193-6639
Chalmers University of Technology · SECentre National de la Recherche Scientifique · FRAstraZeneca (Sweden) · SE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methods for tracking RNA inside living cells without perturbing their natural interactions and functions are critical within biology and, in particular, to facilitate studies of therapeutic RNA delivery. We present a stealth labeling approach that can efficiently, and with high fidelity, generate RNA transcripts, through enzymatic incorporation of the triphosphate of tC

Indexed as

FluorescenceMolecular ImagingCell Line, TumorCOVID-19 Drug TreatmentCytosineFluorescent DyesGreen Fluorescent ProteinsHistonesHumansMolecular StructureRNA, MessengerSpectrometry, FluorescenceCytosineFluorescent DyesGreen Fluorescent ProteinsHistonesRNA, Messenger

Identifiers

PMID33797236
PMCPMC8154517
OpenAlexW3148084463

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.