Evidence map›Paper›PMID 33797013›Full record

ArticleBiochemical genetics2021

Molecular Cloning and Characterization of FAD6 Gene from Chia (Salvia hispanica L.).

Yufei Xue, Lejing Li, Xuemei Liu, Huanhuan Jiang, Yanping Zhao, Songzhen Wei, Na Lin, Yourong Chai

Abstract read
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In one paragraph

Article in Biochemical genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yufei XueCollege of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Lejing LiCollege of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Xuemei LiuCollege of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Huanhuan JiangCollege of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Yanping ZhaoCollege of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Songzhen WeiCollege of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Na LinCollege of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China.
Yourong ChaiCollege of Agronomy and Biotechnology, Chongqing Rapeseed Engineering Research Center, Academy of Agricultural Sciences, Southwest University, Chongqing, 400715, China. chaiyourong2@163.com.ORCID http://orcid.org/0000-0001-6611-624X

Funding

Chongqing Research Program of Basic Research and Frontier Technology cstc2015jcyjBX0143Fundamental Research Funds for the Central Universities XDJK2020C038Ministry of Science and Technology of the People's Republic of China 2016YFD0100506National Natural Science Foundation of China 31871549National Natural Science Foundation of China 32001441
6 · The paper itself

Abstract

Plastidial Δ12 fatty acid desaturase (FAD6) is a key enzyme for linoleic acid (LA) and α-linolenic acid (ALA) biosynthesis. Chia (Salvia hispanica L.) is a revived omega-3 plant source that is richest in ALA level. In this study, based on the RACE method, one full-length cDNA sequence encoding FAD6, named ShFAD6, was isolated from chia. There exist three alternative transcription start sites and five alternative poly(A) tailing sites in ShFAD6. The 5'UTR of ShFAD6 contains a purine-stretch of 44 bp. ShFAD6 has an ORF of 1335 bp encoding a 444 aa protein of 51.33 kDa. ShFAD6 contains a conserved Delta12-FADS-like domain together with three strong trans-membrane helices and three histidine motifs. There also exists a chloroplast transmit peptide in ShFAD6 N-terminal. Phylogenetic analyses validated its identity of dicot FAD6 protein and suggested some critical evolutionary features of plant FAD6 genes. Heterologous yeast expression confirmed the catalytic activity of ShFAD6. The qRT-PCR assay showed that ShFAD6 is mainly expressed in leaves, stems, flowers, buds and early-stage seeds, and also responded to various stresses and hormone treatments. Under Sclerotinia infection, qRT-PCR and fluorescence imaging illustrated the possible correlation of ShFAD6 expression and photosynthesis. This study provides insight for further function study of ShFAD6 in oil quality improvement in staple oilseed crops as well as stress response and adaptation in plants.

Indexed as

Gene Expression Regulation, PlantAmino Acid SequenceAscomycotaBase SequenceCloning, MolecularFatty Acid DesaturasesPhylogenyPlant DiseasesPlant ProteinsSalviaSeedsSequence Homology, Amino AcidFatty Acid DesaturasesPlant ProteinsChia (Salvia hispanica L.)CloningFAD6Transcriptional expressionYeast expression

Identifiers

PMID33797013

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.