Evidence map›Paper›PMID 33796619›Full record

ArticleBio-protocol2021

Strategy of Isolating 'Primed' Tumor Initiating Cells Based on Mitochondrial Transmembrane Potential.

Brian Spurlock, Vidya Sagar Hanumanthu, Kasturi Mitra

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Brian SpurlockDepartment of Genetics, University of Alabama at Birmingham, AL 35294, USA.
Vidya Sagar HanumanthuDivision of Clinical Immunology and Rheumatology, University of Alabama at Birmingham, AL 35294, USA.
Kasturi MitraDepartment of Genetics, University of Alabama at Birmingham, AL 35294, USA.
University of Alabama at Birmingham · US

Funding

RT-SQuARED-M method to study the precise role of mitochondria in the oxidative damage caused by environmental toxinsR33ES025662 · NIEHS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MITRA, KASTURI · 2018 to 2019
$1.1M
RT-SQuARED-M method to study the precise role of mitochondria in the oxidative damage caused by environmental toxinsR21ES025662 · NIEHS · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI MITRA, KASTURI · 2015 to 2017
$549k
NIEHS NIH HHS R21 ES025662NIEHS NIH HHS R33 ES025662
6 · The paper itself

Abstract

Various stem cells have been found to be dependent on mitochondrial energetics. The role of mitochondria in regulating the self-renewal of normal stem cells and stem-like tumor initiating cells (TICs) is increasingly being appreciated. We proposed that TIC populations have a sub population of cells that are "primed" by mitochondria for self-renewal. Using ovarian cancer model, we have developed a protocol to identify and isolate these "primed" cells using Fluorescence-Assisted Cell Sorting (FACS). We combined live cell stains for a functional marker of TICs and for mitochondrial transmembrane potential to enrich TICs with higher mitochondrial potential that form

Indexed as

FACSMitochondrial energeticsSelf-RenewalStem Cell PrimingTumor initiating cells

Identifiers

PMID33796619
PMCPMC8005873
OpenAlexW3134126935

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.