ArticleFrontiers in cellular and infection microbiology2021
Factors Associated With MALDI-TOF Mass Spectral Quality of Species Identification in Clinical Routine Diagnostics.
Article in Frontiers in cellular and infection microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Comparison of Bruker Biotyper Sirius, VITEK MS PRIME, and ASTA MicroIDSys MALDI-TOF MS Systems forAnnals of laboratory medicine · 2026Article
- Validation of a MALDI Intact Cell Mass Spectrometry Workflow for Routine Application in Monitoring Industrial E. coli Fermentations.Journal of mass spectrometry : JMS · 2026Article
- Comparative analytical performance of Autof MS2600 and Biotyper Sirius for rapid MALDI-TOF MS identification of microorganisms from positive blood cultures.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Resolution of MALDI-TOF MS compared to whole genome sequencing for the identification of Vibrio parahaemolyticus strains isolated from oysters.Archives of microbiology · 2026Article
- Evaluating the Autof MS2600 vs. Biotyper Sirius MALDI-TOF MS: a head-to-head study for routine microbial identification in a university clinical microbiology laboratory.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- A Modern Framework for Identifying Novel EnvironmentalLife (Basel, Switzerland) · 2026Article
- Machine Learning-Based Prediction of Antimicrobial Resistance inDiagnostics (Basel, Switzerland) · 2026Article
- Rapid identification of carbapenem-resistant Acinetobacter baumannii based on MALDI-TOF mass spectrometry and machine learning.BMC microbiology · 2026Article
- Observational
- MALDI-TOF MS Classification of Formosan Thraustochytrids and Their Use in Shrimp Farming.Journal of agricultural and food chemistry · 2026Article
- Effect of riboflavin and roseoflavin, a structural analogue of riboflavin, on the growth of selected microbial isolates from human saliva using a riboflavin-free cultivation medium.Folia microbiologica · 2025Article
- Experimental study on factors influencing low identification rates and spectral quality ofMicrobiology spectrum · 2025Article
- MALDI-TOF MS Biomarkers for Methicillin-ResistantMetabolites · 2025Review
- Identification, Comparison, and Profiling of Selected Diarrhoeagenic Pathogens from Diverse Water Sources and Human and Animal Faeces Using Whole-Genome Sequencing.Microorganisms · 2025Article
- Epidemiological insight into bacterial risk associated with flooded areas of city agglomeration (Wrocław, Poland 2024).Scientific reports · 2025Article
- Accurate identification ofJournal of medical microbiology · 2025Article
- A MALDI-ToF mass spectrometry database for identification and classification of highly pathogenic bacteria.Scientific data · 2025Article
- An antimicrobial drug recommender system using MALDI-TOF MS and dual-branch neural networks.eLife · 2024Article
- PredictingmSystems · 2024Article
- Environmental bacterial load during surgical and ultrasound procedures in a Swedish small animal hospital.Acta veterinaria Scandinavica · 2024Article
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Authors and funding
4 authors.
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Abstract
Background: An accurate and timely identification of bacterial species is critical in clinical diagnostics. Species identification allows a potential first adaptation of empiric antibiotic treatments before the resistance profile is available. Matrix assisted Laser Desorption Ionization Time of Flight mass spectrometry (MALDI-TOF MS) is a widely used method for bacterial species identification. However, important challenges in species identification remain. These arise from (i) incomplete databases, (ii) close relatedness of species of interest, and (iii) spectral quality, which is currently vaguely defined. Methods: We selected 47 clinically relevant bacterial isolates from 39 species, which can be challenging to identify by MALDI-TOF MS. We measured these isolates under various analytical conditions on two MALDI-TOF MS systems. First, we identified spectral features, which were associated with correct species identification in three different databases. Considering these features, we then systematically compared spectra produced with three different sample preparation protocols. In addition, we varied quantities of bacterial colony material applied and bacterial colony age. Results: We identified (i) the number of ribosomal marker peaks detected, (ii) the median relative intensity of ribosomal marker peaks, (iii) the sum of the intensity of all detected peaks, (iv) a high measurement precision, and (v) reproducibility of peaks to act as good proxies of spectral quality. We found that using formic acid, measuring bacterial colonies at a young age, and frequently calibrating the MALDI-TOF MS device increase mass spectral quality. We further observed significant differences in spectral quality between different bacterial taxa and optimal measurement conditions vary per taxon. Conclusion: We identified and applied quality measures for MALDI-TOF MS and optimized spectral quality in routine settings. Phylogenetic marker peaks can be reproducibly detected and provide an increased resolution and the ability to distinguish between challenging species such as those within the
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