Evidence map›Paper›PMID 33796137›Full record

ArticleThe journal of venomous animals and toxins including tropical diseases2021

Identification and recombinant expression of an antimicrobial peptide (cecropin B-like) from soybean pest

Luís Felipe Costa Ramos, João Henrique de Oliveira Rangel, Guilherme Caldas Andrade, Carolina Lixa, Livia Vieira Araujo de Castilho, Fábio César Sousa Nogueira, Anderson S Pinheiro, Fabio Mendonça Gomes, Cristiane Dinis AnoBom, Rodrigo Volcan Almeida and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of venomous animals and toxins including tropical diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Luís Felipe Costa RamosDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
João Henrique de Oliveira RangelDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Guilherme Caldas AndradeDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Carolina LixaDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Livia Vieira Araujo de CastilhoDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Fábio César Sousa NogueiraDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Anderson S PinheiroDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Fabio Mendonça GomesCarlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Cristiane Dinis AnoBomDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.
Rodrigo Volcan AlmeidaDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.ORCID 0000-0002-0164-6044
Danielle Maria Perpétua de OliveiraDepartment of Biochemistry, Institute of Chemistry, Center of Mathematical and Natural Sciences, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.ORCID 0000-0002-9889-5556
Universidade Federal do Rio de Janeiro · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

abstract

backgroundInsects can be found in numerous diverse environments, being exposed to pathogenic organisms like fungi and bacteria. Once these pathogens cross insect physical barriers, the innate immune system operates through cellular and humoral responses. Antimicrobial peptides are small molecules produced by immune signaling cascades that develop an important and generalist role in insect defenses against a variety of microorganisms. In the present work, a cecropin B-like peptide (AgCecropB) sequence was identified in the velvetbean caterpillar

methodsAgCecropB sequence (without the signal peptide) was cloned in the plasmid vector pET-M30-MBP and expressed in the

resultsAgCecropB was expressed in

conclusionsThe first cecropin B-like peptide was described in

Indexed as

Agricultural pestAnticarsia gemmatalisAntimicrobial peptidesCecropin BHeterologous expression

Identifiers

PMID33796137
PMCPMC7970720
OpenAlexW3137314064

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.