ArticleNature chemical biology2021
The HaloTag as a general scaffold for far-red tunable chemigenetic indicators.
Article in Nature chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 174 citations in OpenAlex.
- A chemigenetic endogenous Ras activity biosensor for live-cell pharmacology.Nature chemical biology · 2026Article
- CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.Nature neuroscience · 2026Article
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
- Illuminating the Future of Catecholamine Detection.Journal of neurochemistry · 2026Review
- Achieving cell-type-specific bioorthogonal chemistry using enzyme-activated caged tetrazines.Nature chemical biology · 2026Article
- Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter.bioRxiv : the preprint server for biology · 2026Article
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- Dynamic visualization of physiological CaMKII activity using sensitive FRET biosensors.bioRxiv : the preprint server for biology · 2026Article
- NirFAP680: a highly bright and stable large Stokes shift near-infrared fluorogen-activating protein.Nature methods · 2026Article
- Painting dopamine in far-red: a chemigenetic breakthrough.Science China. Life sciences · 2026Article
- A series of spontaneously blinking dyes for super-resolution microscopy.Nature methods · 2026Article
- HaloTag-based approach to quantify subcellular localization of TRPV3 channels.Biophysical journal · 2026Article
- Monitoring in real time and far-red imaging of HNature chemical biology · 2026Article
- Direct Observation of Nanometer-Sized Steps of Single Myosin VI Molecules in Living Cells.Nano letters · 2026Article
- Mechanistic insights into the color transformation of a non-FRET substrate for RNase activity detection.bioRxiv : the preprint server for biology · 2026Article
- Far-red chemigenetic kinase biosensors enable multiplexed and super-resolved imaging of signaling networks.Nature biotechnology · 2026Article
- Steric confinement-induced emission probe for monitoring protein conformations in live cells.Communications chemistry · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Bathochromic Shift via C=O to C=S Substitution: A Far-Red Fluorogen for Multiplexed FLIM with FAST Fluorogen-Activating Protein.International journal of molecular sciences · 2025Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 1 institution in 3 countries.
Funding
Abstract
Functional imaging using fluorescent indicators has revolutionized biology, but additional sensor scaffolds are needed to access properties such as bright, far-red emission. Here, we introduce a new platform for 'chemigenetic' fluorescent indicators, utilizing the self-labeling HaloTag protein conjugated to environmentally sensitive synthetic fluorophores. We solve a crystal structure of HaloTag bound to a rhodamine dye ligand to guide engineering efforts to modulate the dye environment. We show that fusion of HaloTag with protein sensor domains that undergo conformational changes near the bound dye results in large and rapid changes in fluorescence output. This generalizable approach affords bright, far-red calcium and voltage sensors with highly tunable photophysical and chemical properties, which can reliably detect single action potentials in cultured neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.