Evidence map›Paper›PMID 33795833›Full record

ArticleCommunications biology2021

Adhering interacting cells to two opposing coverslips allows super-resolution imaging of cell-cell interfaces.

Julia Sajman, Yair Razvag, Shachar Schidorsky, Seon Kinrot, Kobi Hermon, Oren Yakovian, Eilon Sherman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. High- and Super-Resolution Imaging of Cell-Cell Interfaces.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Julia Sajman *Racah Institute of Physics, The Hebrew University, Jerusalem, Israel.
Yair Razvag *Racah Institute of Physics, The Hebrew University, Jerusalem, Israel.
Shachar SchidorskyRacah Institute of Physics, The Hebrew University, Jerusalem, Israel.
Seon KinrotRacah Institute of Physics, The Hebrew University, Jerusalem, Israel.ORCID http://orcid.org/0000-0001-5614-0983
Kobi HermonRacah Institute of Physics, The Hebrew University, Jerusalem, Israel.
Oren YakovianRacah Institute of Physics, The Hebrew University, Jerusalem, Israel.
Eilon ShermanRacah Institute of Physics, The Hebrew University, Jerusalem, Israel. eilon.sherman@mail.huji.ac.il.ORCID http://orcid.org/0000-0002-7403-6036

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-cell interfaces convey mechanical and chemical information in multicellular systems. Microscopy has revealed intricate structure of such interfaces, yet typically with limited resolution due to diffraction and unfavourable orthogonal orientation of the interface to the coverslip. We present a simple and robust way to align cell-cell interfaces in parallel to the coverslip by adhering the interacting cells to two opposing coverslips. We demonstrate high-quality diffraction-limited and super-resolution imaging of interfaces (immune-synapses) between fixed and live CD8

Indexed as

CD8-Positive T-LymphocytesCost-Benefit AnalysisMicroscopy

Identifiers

PMID33795833
PMCPMC8016881

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.